Morphological and structural response of Bacillus sp. SFC 500-1E after Cr(VI) and phenol treatment
| Autor: | Paola S. González, Gustavo M. Morales, Natalia Soledad Paulucci, Elizabeth Agostini, Eugenia Reynoso, Marilina Fernandez |
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| Rok vydání: | 2020 |
| Předmět: |
Chromium
Lysis Bacillus Applied Microbiology and Biotechnology Cell membrane 03 medical and health sciences chemistry.chemical_compound Stress Physiological medicine Extracellular Phenol Phospholipids Unsaturated fatty acid 030304 developmental biology 0303 health sciences Chromatography Strain (chemistry) Extracellular Polymeric Substance Matrix 030306 microbiology Chemistry Cell Membrane Fatty Acids Biofilm General Medicine Biodegradation Environmental medicine.anatomical_structure Biofilms Saturated fatty acid |
| Zdroj: | Journal of Basic Microbiology. 60:679-690 |
| ISSN: | 0233-111X |
| Popis: | Bacillus sp. SFC 500-1E, a bacterial strain isolated from tannery sediments, is able to remove Cr(VI) and simultaneously tolerate high concentrations of phenol. In this study, we used high-resolution microscopies, fluorescence polarization techniques, and several biochemical approaches to improve our understanding about the adaptive mechanisms of this strain to survive in the presence of Cr(VI) and phenol, both individually and simultaneously. Among adaptive strategies developed by Bacillus sp. SFC 500-1E, an increase in bacterial size, such as length, width, and height, and ultrastructural alterations, such as electron-dense precipitates, the presence of exopolymers, and cell lysis, are noteworthy. The exopolymers observed were consistent with the extensive biofilm formation and exopolysaccharides and extracellular protein quantification. At the cell membrane level, a rapid rigidity was induced in Cr(VI) + phenol treatment. This effect was counteracted after 16 h by changes at the level of phospholipids, mainly in the composition of fatty acids (FAs); in particular, an increase in the unsaturated fatty acid/saturated fatty acid ratio was detected. This study shows evidence of some adaptive responses displayed by Bacillus sp. SFC 500-1E, which allows it to survive in stressful conditions. |
| Databáze: | OpenAIRE |
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