Antineoplastic effects of Chlorella pyrenoidosa in the breast cancer model
| Autor: | Bianka Bojková, Dusan Dobrota, Desanka Výbohová, Peter Kružliak, Peter Kubatka, Martina Chripkova, Andrea Kapinová, Marian Adamkov, Miroslav Novák, Karol Kajo, Martin Kello, Nadežda Stollárová, Ján Mojžiš, Monika Kassayová, Martin Péč |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Vascular Endothelial Growth Factor A
Pathology medicine.medical_specialty Angiogenesis Endocrinology Diabetes and Metabolism Apoptosis Breast Neoplasms Chlorella Pharmacology medicine.disease_cause Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo medicine Microalgae Chlorella pyrenoidosa Animals Humans Annexin A5 030304 developmental biology Cell Proliferation Caspase 7 Membrane Potential Mitochondrial 0303 health sciences Nutrition and Dietetics biology Cancer biology.organism_classification medicine.disease Antineoplastic Agents Phytogenic 3. Good health Diet MCF-7 030220 oncology & carcinogenesis MCF-7 Cells Female Carcinogenesis Reactive Oxygen Species Phytotherapy |
| Zdroj: | Nutrition (Burbank, Los Angeles County, Calif.). 31(4) |
| ISSN: | 1873-1244 |
| Popis: | Objectives: There has been considerable interest in both clinical and preclinical research about the role of phytochemicals in the reduction of risk for cancer in humans. The aim of this study was to determine the antineoplastic effects of Chlorella pyrenoidosa in experimental breast cancer in vivo and in vitro. Methods: In this experiment, the antineoplastic effects of Chlorella pyrenoidosa in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Chlorella powder was administered through diet at concentrations of 0.3% and 3%. The experiment was terminated 14 wk after carcinogen administration. At autopsy, mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. In vitro cytotoxicity assay, parameters of apoptosis, and proliferation after chlorella treatment in human breast adenocarcinoma (MCF-7) cells were carried out. Results: Basic parameters of experimental carcinogenesis, mechanism of action (biomarkers of apoptosis, proliferation, and angiogenesis), chosen metabolic variables, and side effects after long-term chlorella treatment in animals were assessed. Chlorella at higher concentration suppressed tumor frequency by 61% (P < 0.02) and lengthened tumor latency by 12.5 d (P < 0.02) in comparison with the controls. Immunohistochemical analysis of rat tumor cells showed caspase-7 expression increase by 73.5% (P < 0.001) and vascular endothelial growth factor receptor-2 expression decrease by 19% (P = 0.07) after chlorella treatment. In a parallel in vitro study, chlorella significantly decreased survival of MCF-7 cells in a dose-dependent manner. In chlorella-treated MCF-7 cells, a significant increase in cells having sub-G(0)/G(1) DNA content and significant increase of early apoptotic and late apoptotic/necrotic cells after annexin V/PI staining assay were found. Decreases in mitochondrial membrane potential and increasing reactive oxygen species generation were observed in the chlorella-treated MCF-7 cells. Conclusions: This study is the first report on the antineoplastic effects of Chlorella pyrenoidosa in experimental breast cancer in vivo and in vitro. (C) 2015 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect