Loss of the Extracellular Matrix Protein DIG-1 Causes Glial Fragmentation, Dendrite Breakage, and Dendrite Extension Defects
| Autor: | Megan K. Chong, Karolina Mizeracka, Maxwell G. Heiman, Elizabeth R. Cebul |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nervous system
neurodevelopment QH301-705.5 Chemistry dendrites glia extracellular matrix Morphogenesis Amphid Dendrite Cell Biology C. elegans Article Cell biology Extracellular matrix medicine.anatomical_structure DIG-1 Sensory dendrite medicine Dendrite extension Biology (General) Axon Molecular Biology Developmental Biology |
| Zdroj: | Journal of Developmental Biology Journal of Developmental Biology, Vol 9, Iss 42, p 42 (2021) Volume 9 Issue 4 |
| ISSN: | 2221-3759 |
| Popis: | The extracellular matrix (ECM) guides and constrains the shape of the nervous system. InC. elegans, DIG-1 is a giant ECM component that is required for fasciculation of sensory dendrites during development and for maintenance of axon positions throughout life. We identified four novel alleles ofdig-1in three independent screens for mutants affecting disparate aspects of neuronal and glial morphogenesis. First, we find that disruption of DIG-1 causes fragmentation of the amphid sheath glial cell in larvae and young adults. Second, it causes severing of the BAG sensory dendrite from its terminus at the nose tip, apparently due to breakage of the dendrite as animals reach adulthood. Third, it causes embryonic defects in dendrite fasciculation in inner labial (IL2) sensory neurons, as previously reported, as well as rare defects in IL2 dendrite extension that are enhanced by loss of the apical ECM component DYF-7, suggesting that apical and basolateral ECM contribute separately to dendrite extension. Our results highlight novel roles for DIG-1 in maintaining the cellular integrity of neurons and glia, possibly by creating a barrier between structures in the nervous system. |
| Databáze: | OpenAIRE |
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