Connexin43 Suppresses Lung Cancer Stem Cells
| Autor: | Randall J. Ruch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Cell signaling cancer stem cell connexin Connexin Biology lcsh:RC254-282 Article 03 medical and health sciences 0302 clinical medicine Cancer stem cell medicine Neoplastic transformation Lung cancer gap junctions Large cell cell communication lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease lung cancer 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Adenocarcinoma sense organs Stem cell |
| Zdroj: | Cancers Cancers, Vol 11, Iss 2, p 175 (2019) Volume 11 Issue 2 |
| ISSN: | 2072-6694 |
| Popis: | Alterations in gap junctions and their protein components, connexins, have been associated with neoplastic transformation and drug resistance, and more recently have been shown to play important roles in cancer stem cells (CSCs). However, there is less knowledge of connexins and gap junctions in lung CSCs. To address this, Connexin43 (Cx43), the major human lung epithelial gap junction protein, was expressed ectopically in poorly expressing National Cancer Institute-125 (NCI-H125) metastatic human lung adenocarcinoma cells, and phenotypic characteristics of malignant cells and abundance of CSCs were evaluated. The ectopic expression of Cx43 resulted in the formation of functional gap junctions a more epithelial morphology reduced proliferation, invasion, colony formation, tumorsphere formation, pluripotency marker expression, and percentage of aldehyde dehydrogenase (ALDH)-positive cells and increased cisplatin sensitivity. Similarly, in NCI-H522 (human lung adenocarcinoma) and NCI-H661 (human lung large cell carcinoma) cell lines, which express Cx43 and functional gap junctions endogenously, the Cx43 content was lower in tumorspheres and ALDH-positive cells than in bulk cells. These results demonstrate that Cx43 can reverse several neoplastic characteristics and reduce the abundance of human lung CSCs. |
| Databáze: | OpenAIRE |
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