Premature contractions of the bladder are suppressed by interactions between TRPV4 and SK3 channels in murine detrusor PDGFRα+ cells
| Autor: | Kenton M. Sanders, Robert D. Corrigan, Brian A. Perrino, Byoung H. Koh, Toby C. Chai, Haeyeong Lee, Lauren E. Peri, Hyun-Tai Lee, Sang Don Koh, Nikita E. George, Yeming Xie, Bhupal P. Bhetwal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
TRPV4 Agonist Detrusor muscle Receptor Platelet-Derived Growth Factor alpha medicine.drug_class Small-Conductance Calcium-Activated Potassium Channels Urinary Bladder lcsh:Medicine TRPV Cation Channels urologic and male genital diseases Article SK channel 03 medical and health sciences Mice 0302 clinical medicine SK3 medicine Animals lcsh:Science Cells Cultured Muscle Cells Multidisciplinary Urinary bladder Chemistry lcsh:R Hyperpolarization (biology) female genital diseases and pregnancy complications 3. Good health Cell biology 030104 developmental biology medicine.anatomical_structure Mechanosensitive channels lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
| ISSN: | 2045-2322 |
| Popis: | During filling, urinary bladder volume increases dramatically with little change in pressure. This is accomplished by suppressing contractions of the detrusor muscle that lines the bladder wall. Mechanisms responsible for regulating detrusor contraction during filling are poorly understood. Here we describe a novel pathway to stabilize detrusor excitability involving platelet-derived growth factor receptor-α positive (PDGFRα+) interstitial cells. PDGFRα+ cells express small conductance Ca2+-activated K+ (SK) and TRPV4 channels. We found that Ca2+ entry through mechanosensitive TRPV4 channels during bladder filling stabilizes detrusor excitability. GSK1016790A (GSK), a TRPV4 channel agonist, activated a non-selective cation conductance that coupled to activation of SK channels. GSK induced hyperpolarization of PDGFRα+ cells and decreased detrusor contractions. Contractions were also inhibited by activation of SK channels. Blockers of TRPV4 or SK channels inhibited currents activated by GSK and increased detrusor contractions. TRPV4 and SK channel blockers also increased contractions of intact bladders during filling. Similar enhancement of contractions occurred in bladders of Trpv4−/− mice during filling. An SK channel activator (SKA-31) decreased contractions during filling, and rescued the overactivity of Trpv4−/− bladders. Our findings demonstrate how Ca2+ influx through TRPV4 channels can activate SK channels in PDGFRα+ cells and prevent bladder overactivity during filling. |
| Databáze: | OpenAIRE |
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