Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines
| Autor: | Jamin A. Willoughby, Heidi Baas, William R. Blakemore, James M. McKim, Gabriel P. Rice, Myra L. Weiner |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Membrane Permeability Blotting Western Apoptosis Toxicology medicine.disease_cause Carrageenan Real-Time Polymerase Chain Reaction Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Intestinal Neoplasms Tumor Cells Cultured Medicine Humans RNA Messenger Cytotoxicity Cell Proliferation business.industry Cell growth Reverse Transcriptase Polymerase Chain Reaction Liver cell Liver Neoplasms 04 agricultural and veterinary sciences General Medicine 040401 food science Molecular biology Gene Expression Regulation Neoplastic 030104 developmental biology chemistry Immunology TLR4 Cytokines Inflammation Mediators business Oxidative stress Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 96 |
| ISSN: | 1873-6351 |
| Popis: | Carrageenan (CGN) is a common food additive used for its gelling and thickening properties. The present study was done to evaluate intestinal permeability, cytotoxicity, and CGN-mediated induction of proinflammatory cytokines. A standard Caco-2 absorption model showed no CGN permeability or cytotoxicity at concentrations of 100, 500, and 1000 μg/mL. In two human intestinal cell lines (HT-29 and HCT-8) CGN (0.1, 1.0, and 10.0 μg/mL) did not induce IL-8, IL-6, or MCP-1 (CCL2) or produce cellular toxicity after 24 h. The TLR4 agonist LPS produced weak induction of IL-8 in HT-29 cells and no induction in HCT-8 cells. The effects of κ-CGN (0.1, 1.0, and 10 μg/mL) on cellular oxidative stress was assessed in HT-29 cells using CM-H2DCFDA as the probe. No effect on oxidative stress was observed after 24 h. In the human (HepG2) liver cell line, ʎ-CGN (0.1, 1.0, 10.0 and 100.0 μg/mL) had no effect on the expression of IL-8, IL-6, or MCP-1 (CCL2) after 24 h. In conclusion, CGN was not absorbed, and was not cytotoxic. It did not induce oxidative stress, and did not induce proinflammatory proteins. |
| Databáze: | OpenAIRE |
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