Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways
| Autor: | Qiao-yan Zhang, Meng-qin Liu, Bo Zhu, Lu-lin Zhu, Qi Zhang, Quan-long Zhang, Lu-ping Qin, Yu-qiong He, Hai-Liang Xin, Yi Shen, Jian-hua Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MAPK/ERK pathway
Male China medicine.drug_class MAP Kinase Signaling System Anti-Inflammatory Agents Pharmacology Plant Roots Anti-inflammatory NF-κB Morinda officinalis Proinflammatory cytokine Arthritis Rheumatoid 03 medical and health sciences Mice 0302 clinical medicine Western blot RAW 264.7 macrophages In vivo Iridoid glycoside Anti-inflammation medicine Animals Morinda Rats Wistar Anti-arthritis 030304 developmental biology 0303 health sciences Analgesics biology medicine.diagnostic_test Dose-Response Relationship Drug Chemistry Plant Extracts NF-kappa B MAPK pathway lcsh:Other systems of medicine biology.organism_classification lcsh:RZ201-999 Acute toxicity Rats RAW 264.7 Cells Complementary and alternative medicine Toxicity Iridoid Glycosides Morinda officinalis how 030217 neurology & neurosurgery Research Article |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-14 (2020) BMC Complementary Medicine and Therapies |
| ISSN: | 2662-7671 |
| Popis: | Background The root of Morinda officinalis How. (MO, the family of Rubiaceae) has long been used to treat inflammatory diseases in China and other eastern Asian countries, and iridoid glycosides extracted from MO (MOIG) are believed to contribute to this anti-inflammatory effect. However, the mechanism underlying the anti-inflammatory and anti-arthritic activities of MOIG has not been elucidated. The aim of the present study was to determine how MOIG exerted anti-inflammatory and anti-arthritic effects in vivo and in RAW 264.7 macrophages. Methods MOIG were enriched by XDA-1 macroporous resin. The maximum feasible dose method was adopted to evaluate its acute toxicity. The analgesic effect of MOIG was evaluated by acetic acid writhing test and the anti-inflammatory effect was evaluated by cotton-pellet granuloma test in rats and air pouch granuloma test in mice. The anti-arthritic effect was evaluated by establishing an adjuvant arthritis model induced by Complete Freund’s Adjuvant (CFA). The viability of the cultured RAW 264.7 macrophages was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The anti-inflammatory activity was evaluated by measuring NO, IL-1β, IL-6 and TNF-α levels in LPS-stimulated RAW 264.7 cells. The protein level of inflammatory responsive genes was evaluated by Western blot analysis. Results MOIG had no significant toxicity at maximum feasible dose of 22.5 g/kg. MO extracts and MOIG (50,100 and 200 mg/kg) all evoked a significantly inhibitory effects on the frequency of twisting induced by acetic acid in mice compared with the model control group. Administration of MO extracts and MOIG markedly decreased the dry and wet weight of cotton pellet granuloma in rats and air pouch granuloma in mice. MOIG significantly attenuated the paw swelling and decreased the arthritic score, weight loss, spleen index, and the serum level of inflammatory factors IL-1β, IL-6 and IL-17a in CFA-induced arthritic rats. MOIG inhibited the production of inflammatory cytokines in LPS-stimulated RAW264.7 cells, and the expressions of iNOS, COX-2 and proteins related to MAPK and NF-κB signaling pathways in LPS-stimulated RAW 264.7 macrophages. Conclusion MOIG exerted anti-inflammatory and anti-arthritic activities through inactivating MAPK and NF-κB signaling pathways, and this finding may provide a sound experimental basis for the clinical treatment of rheumatoid arthritis with MOIG. |
| Databáze: | OpenAIRE |
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