A naturally occurring soluble isoform of murine Fas generated by alternative splicing
| Autor: | D. P. M. Hughes, I. N. Crispe |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Fas Ligand Protein
RNA Splicing Molecular Sequence Data Immunology Population Mast-Cell Sarcoma Apoptosis Biology Lymphocyte Activation Transfection Fas ligand Mice Exon Tumor Cells Cultured Animals Immunology and Allergy Amino Acid Sequence RNA Messenger fas Receptor education education.field_of_study Membrane Glycoproteins Base Sequence Alternative splicing Exons Articles Fas receptor Molecular biology Lymphoproliferative Disorders Recombinant Proteins Mice Inbred C57BL Liver Solubility RNA splicing |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.182.5.1395 |
| Popis: | We report a soluble isoform of mouse Fas, which is generated by alternative splicing of Fas mRNA to a newly identified exon located between exons 2 and 3 of the previously published Fas sequence. This splicing event creates a novel Fas transcript, Fas beta, with the potential to encode a truncated form of the extracellular domain, termed Fas B. In vitro, P815 mastocytoma cells transfected with Fas B become resistant to Fas ligand-induced apoptosis, and the resistance is mediated by a secreted product of the transfected cells. In vivo, Fas beta mRNA expression is correlated inversely with apoptosis among subsets of intrahepatic T lymphocytes, a cell population in which activation-induced T cell apoptosis occurs. We propose that Fas B is a new cytokine that acts physiologically to limit apoptosis induced by Fas ligand. |
| Databáze: | OpenAIRE |
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