Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering
| Autor: | Mihaela Crisan, Greg Asatrian, Erin Zou, Venu Lagishetty, Winters R. Hardy, Kang Ting, Xinli Zhang, Pei Liang, Sonja Lobo, Catherine Ding, Martin K. Childers, Carolyn A. Meyers, Bruno Péault, Yu Hsin Yen, Aaron W. James, Chia Soo |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Pathology Physiology Cellular differentiation CD34 Adipose tissue lcsh:Medicine Alizarin Staining Cell Separation Spectrum Analysis Techniques Endocrinology Tissue engineering Osteogenesis Animal Cells Medicine and Health Sciences Group-Specific Staining Enzyme-Linked Immunoassays lcsh:Science Cells Cultured Platelet-Derived Growth Factor Staining Fluorescence-Activated Cell Sorting Multidisciplinary Stem Cells digestive oral and skin physiology Cell Differentiation Recombinant Proteins Adipose Tissue Spectrophotometry CD146 Engineering and Technology Fibroblast Growth Factor 2 Cytophotometry Anatomy Cellular Types Research Article Biotechnology medicine.medical_specialty Stromal cell Nerve Tissue Proteins Bioengineering Biology Research and Analysis Methods Bone and Bones 03 medical and health sciences Dogs Growth Factors medicine Journal Article Animals Humans Progenitor cell Immunoassays Tissue Engineering Endocrine Physiology Mesenchymal stem cell lcsh:R Calcium-Binding Proteins Biology and Life Sciences Mesenchymal Stem Cells Cell Biology digestive system diseases 030104 developmental biology Biological Tissue Specimen Preparation and Treatment Immunologic Techniques lcsh:Q Stromal Cells Pericytes |
| Zdroj: | James, A W, Zhang, X, Crisan, M, Hardy, W R, Liang, P, Meyers, C A, Lobo, S, Lagishetty, V, Childers, M K, Asatrian, G, Ding, C, Yen, Y-H, Zou, E, Ting, K, Peault, B & Soo, C 2017, ' Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering ', PLoS ONE, vol. 12, no. 5, pp. e0177308 . https://doi.org/10.1371/journal.pone.0177308 PLoS ONE PLoS ONE, Vol 12, Iss 5, p e0177308 (2017) |
| Popis: | For over 15 years, human subcutaneous adipose tissue has been recognized as a rich source of tissue resident mesenchymal stem/stromal cells (MSC). The isolation of perivascular progenitor cells from human adipose tissue by a cell sorting strategy was first published in 2008. Since this time, the interest in using pericytes and related perivascular stem/stromal cell (PSC) populations for tissue engineering has significantly increased. Here, we describe a set of experiments identifying, isolating and characterizing PSC from canine tissue (N = 12 canine adipose tissue samples). Results showed that the same antibodies used for human PSC identification and isolation are cross-reactive with canine tissue (CD45, CD146, CD34). Like their human correlate, canine PSC demonstrate characteristics of MSC including cell surface marker expression, colony forming unit-fibroblast (CFU-F) inclusion, and osteogenic differentiation potential. As well, canine PSC respond to osteoinductive signals in a similar fashion as do human PSC, such as the secreted differentiation factor NEL-Like Molecule-1 (NELL-1). Nevertheless, important differences exist between human and canine PSC, including differences in baseline osteogenic potential. In summary, canine PSC represent a multipotent mesenchymogenic cell source for future translational efforts in tissue engineering. |
| Databáze: | OpenAIRE |
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