Real-time monitoring of the metabolic capacity of ex vivo rat olfactory mucosa by proton transfer reaction mass spectrometry (PTR-MS)
| Autor: | Philippe Faure, Aline Robert-Hazotte, Rachel Schoumacker, Jean-Marie Heydel, Jean-Luc Le Quéré |
|---|---|
| Přispěvatelé: | Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Regional Council of Burgundy European Regional Development Fund (FEDER) Carnot Institute Qualiment INRA (QualigrasPhy project) Giract, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Metabolite Ethyl acetate Analytical chemistry Olfaction Acetates Mass spectrometry Biochemistry Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Olfactory mucosa Organ Culture Techniques 0302 clinical medicine Olfactory Mucosa medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Rats Wistar [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology Proton-transfer-reaction mass spectrometry Monitoring Physiologic Volatile Organic Compounds Chromatography Ethanol PTR-MS 030104 developmental biology medicine.anatomical_structure chemistry Odorants XMEs Rat Protons 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry, Springer Verlag, 2016, 408 (6), pp.1539-1543. ⟨10.1007/s00216-015-9289-7⟩ Analytical and Bioanalytical Chemistry, Springer Verlag, 2016, 408 (6), pp.1539-1543. 〈http://link.springer.com/article/10.1007%2Fs00216-015-9289-7〉. 〈10.1007/s00216-015-9289-7〉 |
| ISSN: | 1618-2642 1618-2650 |
| Popis: | International audience; Olfactory mucosa (OM) can metabolise odorant volatile organic compounds through various enzymatic mechanisms to produce odorous or non-odorous metabolites. Preliminary ex vivo studies using headspace-gas chromatography (HS-GC) revealed the formation of metabolites when odorant molecules were injected in the headspace above a fresh explant of rat olfactory mucosa. However, this method did not allow accessing the data during the first 5 min of contact between the odorant and the mucosa; thus limiting the olfactory biological significance. Using a direct-injection mass spectrometry technique with a proton transfer reaction instrument (PTR-MS), we have been able, for the first time, to investigate the first moments of the enzymatic process of the metabolic capacity of ex vivo rat olfactory mucosa in real time. Using ethyl acetate as a model volatile odorous substrate, we demonstrated here for the first time that this odorant could be metabolised by an ex vivo olfactory mucosa within seconds, producing ethanol as metabolite. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink