Longitudinal transkingdom gut microbial approach towards decompensation in outpatients with cirrhosis
| Autor: | Jasmohan S Bajaj, Marcela Peña-Rodriguez, Alex La Reau, Wendy Phillips, Michael Fuchs, Brian C Davis, Richard K Sterling, Masoumeh Sikaroodi, Andrew Fagan, Amirhossein Shamsaddini, Zachariah Henseler, Tonya Ward, Puneet Puri, Hannah Lee, Patrick M Gillevet |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Gut. 72:759-771 |
| ISSN: | 1468-3288 0017-5749 |
| Popis: | ObjectiveFirst decompensation development is a critical milestone that needs to be predicted. Transkingdom gut microbial interactions, including archaeal methanogens, may be important targets and predictors but a longitudinal approach is needed.DesignCirrhosis outpatients who provided stool twice were included. Group 1: compensated, group 2: 1 decompensation (decomp), group 3: >1 decompensationwere followed and divided into those who remained stable or decompensated. Bacteria, viral and archaeal presence, α/β diversity and taxa changes over time adjusted for clinical variables were analysed. Correlation networks between kingdoms were analysed.Results157 outpatients (72 group 1, 33 group 2 and 52 group 3) were followed and 28%–47% developed outcomes. Baseline between those who remained stable/developed outcome: While no α/β diversity differences were seen, commensals were lower and pathobionts were higher in those who decompensated. After decompensation: those experiencing their first decompensation showed greater decrease in α/β-diversity, bacterial change (↑Lactobacillusspp,Streptococcus parasanguinisand ↓beneficial LachnospiraceaeandEubacterium hallii) and viral change (↑Siphoviridae, ↓ Myoviridae) versus those with further decompensation. Archaea: 19% hadMethanobacter brevii, which wassimilar between/within groups. Correlation networks: Baseline archaeal-viral-bacterial networks were denser and more homogeneous in those who decompensated versus the rest. Archaea-bacterial correlations collapsed post first decompensation.Lactobacillusphage Lc Nu and C2-like viruses were negatively linked with beneficial bacteria.ConclusionIn this longitudinal study of cirrhosis outpatients, the greatest transkingdom gut microbial changes were seen in those reaching the first decompensation, compared with subsequent decompensating events. A transkingdom approach may refine prediction and provide therapeutic targets to prevent cirrhosis progression. |
| Databáze: | OpenAIRE |
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