Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
| Autor: | You-Jin Jeon, Duong Thi Thuy Dinh, Youngheun Jee, Hyo Jin Kim, Jinhee Cho, Hyun-Soo Kim, Kalahe Hewage Iresha Nadeeka Madushani Herath, Ginnae Ahn |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Polyphenol
Ovalbumin medicine.medical_treatment Intraperitoneal injection Population Pharmaceutical Science Context (language use) RM1-950 GATA3 Transcription Factor CD3+ CD4+ T cell Andrology Mice Th2 Cells Drug Discovery NLR Family Pyrin Domain-Containing 3 Protein medicine Splenocyte STAT5 Transcription Factor Cytotoxic T cell Animals education Pharmacology education.field_of_study Mice Inbred BALB C biology Dose-Response Relationship Drug Chemistry Plant Extracts Sargassum Interleukin Polyphenols General Medicine Complementary and alternative medicine IL-13 Interleukin 13 biology.protein Molecular Medicine Cytokines Therapeutics. Pharmacology Spleen Research Article |
| Zdroj: | Pharmaceutical Biology article-version (VoR) Version of Record Pharmaceutical Biology, Vol 59, Iss 1, Pp 1464-1472 (2021) |
| ISSN: | 1744-5116 1388-0209 |
| Popis: | Context Sargassum horneri (Turner) C. Agardh (Sargassaceae) is a brown marine alga used in oriental medicine to treat allergic conditions. Objective This study clarifies the effect of polyphenol-containing S. horneri ethanol extract (SHE) on T-helper type-2 (Th2) polarisation. Materials and methods All mice (BALB/c mice, n = 12) except in the healthy control group were first sensitised with an intraperitoneal injection of ovalbumin (OVA; 20 µg) and alum (2 mg) on Day 0 and Day 14. Similarly, phosphate-buffered saline (PBS) was injected according to the same schedule into the healthy control mice. After the final administration, splenocytes were obtained. OVA sensitised mice were challenged with OVA (100 µg/mL) in the absence or presence (62.5 and 125 µg/mL) of SHE while healthy control group remained untreated. Results SHE (0-1000 µg/mL) was not cytotoxic to splenocytes and demonstrated IC50 values of 3.27 and 3.92 mg/mL, respectively, at 24 and 48 h of incubation. SHE suppressed cell proliferation at concentrations ≥62.5 µg/mL. SHE treatment (125 µg/mL) subdued (by 1.8-fold) the population expansion of CD3+CD4+ helper T cells induced by OVA challenge. SHE attenuated the OVA-induced activation of respective transcription factors GATA3 and NLRP3. Simultaneously, highly elevated levels of cytokines interleukin (IL)-4 and IL-5 caused by OVA stimulation were removed completely and IL-13 suppressed by 1.5-fold. Conclusions SHE exhibits Th2 immune suppression under OVA stimulation via GATA3- and NLRP3-dependent IL-4, IL-5, and IL-13 suppression. Therefore, SHE could be therapeutically useful for alleviating the symptoms of allergen-mediated immune diseases. |
| Databáze: | OpenAIRE |
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