Dimethyl Sulfoxide-Free Cryopreservation of Human Umbilical Cord Mesenchymal Stem Cells Based on Zwitterionic Betaine and Electroporation
| Autor: | Ping Ma, Linsheng Zhan, Qianqian Zhou, Yulong Zhang, Lei Zhang, Jing Yang, Liping Lv, Sujing Sun, Min Liu, Lei Gao, Xiaohui Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cryoprotectant QH301-705.5 02 engineering and technology Article Catalysis Cryopreservation Umbilical Cord Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents Betaine Humans Dimethyl Sulfoxide betaine Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Cells Cultured Spectroscopy Cell Proliferation reactive oxygen species molecule delivery Lipotropic Agents Chemistry Dimethyl sulfoxide Electroporation cryoprotectant Organic Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine bioluminescence imaging 021001 nanoscience & nanotechnology Computer Science Applications Cell biology Freezing point Transplantation 030104 developmental biology 0210 nano-technology |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 14 International Journal of Molecular Sciences, Vol 22, Iss 7445, p 7445 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22147445 |
| Popis: | The effective cryopreservation of mesenchymal stem cells (MSCs) is indispensable to the operation of basic research and clinical transplantation. The prevalent protocols for MSC cryopreservation utilize dimethyl sulfoxide (DMSO), which is easily permeable and able to protect MSCs from cryo-injuries, as a primary cryoprotectant (CPA). However, its intrinsic toxicity and adverse effects on cell function remain the bottleneck of MSC cryopreservation. In this work, we cryopreserved human umbilical cord mesenchymal stem cells (UCMSCs) using zwitterionic betaine combined with electroporation without any addition of DMSO. Betaine was characterized by excellent compatibility and cryoprotective properties to depress the freezing point of pure water and balance the cellular osmotic stress. Electroporation was introduced to achieve intracellular delivery of betaine, intending to further provide comprehensive cryoprotection on UCMSCs. Compared with DMSO cryopreservation, UCMSCs recovered from the protocol we developed maintained the normal viability and functions and reduced the level of reactive oxygen species (ROS) that are harmful to cell metabolism. Moreover, the in vivo distribution of thawed UCMSCs was consistent with that of fresh cells monitored by a bioluminescence imaging (BLI) system. This work opens a new window of opportunity for DMSO-free MSC cryopreservation using zwitterionic compounds like betaine combined with electroporation. |
| Databáze: | OpenAIRE |
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