A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry–Based Glycoproteomics
| Autor: | Sharon J. Pitteri, Carolyn R. Bertozzi, Nicholas M. Riley |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
SAX strong anion exchange Special Issue: Glycoproteomics enrichment Glycosylation Computer science DIA data-independent acquisition ConA concanavalin A Review PTMs post-translational modifications Biochemistry Mass Spectrometry Analytical Chemistry ETD electron transfer dissociation IMAC immobilized metal affinity chromatography ManNAz N-azidoacetylmannosamine ZIC-HILIC zwitterionic HILIC Lectins Data-independent acquisition Glycomics Siglecs sialic acid–binding immunoglobulin-type lectins WAX weak anion exchange Chromatography 0303 health sciences 030302 biochemistry & molecular biology glycopeptides PGC porous graphitic carbon Glycoproteomics Graphitic carbon Graphite M6P mannose-6-phosphate strong anion exchange electrostatic repulsion hydrophilic interaction chromatography (SAX-ERLIC) ECD electron capture dissociation Glycoside Hydrolases Ion-mobility spectrometry WGA wheat germ agglutinin Chemical biology chemical biology Computational biology Mass spectrometry affinity chromatography HILIC hydrophilic interaction chromatography 03 medical and health sciences Animals Humans glycoproteomics Molecular Biology hydrophilic interaction chromatography (HILIC) Glycoproteins 030304 developmental biology AAL Aleuria aurantia lectin SPE solid-phase extraction AX anion exchange IMS ion mobility spectrometry MOAC metal oxide affinity chromatography LAC lectin affinity chromatography Neu5Gc N-glycolylneuraminic acid Neu5Ac N-acetylneuraminic acid RCA ricinus communis agglutinin Chemical coupling MS mass spectrometry HCD higher-energy collisional dissociation M-LAC multi-lectin affinity chromatography GalT1 β-1 4-galactosyltransferase 1 Bioorthogonal chemistry CuAAC i.e. “click” chemistry copper-catalyzed azide-alkyne cycloadditions SPAAC i.e. “copper-free click” chemistry strain-promoted azide-alkyne cycloaddition ERLIC electrostatic repulsion-hydrophilic interaction chromatography MAX mixed-mode strong anion exchange |
| Zdroj: | Molecular & Cellular Proteomics : MCP |
| ISSN: | 1535-9476 |
| DOI: | 10.1074/mcp.r120.002277 |
| Popis: | Glycosylation is a prevalent, yet heterogeneous modification with a broad range of implications in molecular biology. This heterogeneity precludes enrichment strategies that can be universally beneficial for all glycan classes. Thus, choice of enrichment strategy has profound implications on experimental outcomes. Here we review common enrichment strategies used in modern mass spectrometry–based glycoproteomic experiments, including lectins and other affinity chromatographies, hydrophilic interaction chromatography and its derivatives, porous graphitic carbon, reversible and irreversible chemical coupling strategies, and chemical biology tools that often leverage bioorthogonal handles. Interest in glycoproteomics continues to surge as mass spectrometry instrumentation and software improve, so this review aims to help equip researchers with the necessary information to choose appropriate enrichment strategies that best complement these efforts. Graphical Abstract Highlights • Glycosylation is complex and often requires enrichment prior to analysis • Review of common enrichment strategies for mass spectrometry–based glycoproteomics • Enrichment methods have practical considerations and experimental implications • Appropriate enrichment strategies will complement developments in mass spectrometry In Brief Interest in mass spectrometry–based glycoproteomics analysis is increasing because of recent advances in instrumentation and data analysis tools. Such studies can provide a wealth of information across a wide spectrum of glycan classes and biological systems. However, many studies require the choice of an enrichment strategy for glycosylated species prior to analysis to obtain the maximum amount of analytical information. Here, common enrichment strategies are reviewed with strengths and weaknesses, and the practical considerations for various methods are discussed. |
| Databáze: | OpenAIRE |
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