TAK1 Participates in c-Jun N-Terminal Kinase Signaling during Drosophila Development
| Autor: | Kunihiro Matsumoto, Hiroshi Shibuya, Mark Stapleton, Makoto Nakamura, Maria C. Danos, Naoto Ueno, Yoshihiro Takatsu, Michael B. O'Connor |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Transgene
Molecular Sequence Data Apoptosis Eye Animals Genetically Modified Mice Transforming Growth Factor beta Animals Drosophila Proteins Amino Acid Sequence Phosphorylation Cell Growth and Development Molecular Biology Phylogeny Genes Dominant Sequence Homology Amino Acid biology MAP kinase kinase kinase c-jun JNK Mitogen-Activated Protein Kinases Cell Biology Transforming growth factor beta MAP Kinase Kinase Kinases biology.organism_classification Molecular biology Cell biology Phenotype Protein kinase domain Microscopy Electron Scanning biology.protein Drosophila Mitogen-Activated Protein Kinases Signal transduction Drosophila melanogaster Drosophila Protein Plasmids Signal Transduction |
| Zdroj: | Molecular and Cellular Biology. 20:3015-3026 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.20.9.3015-3026.2000 |
| Popis: | Transforming growth factor beta (TGF-beta)-activated kinase 1 (TAK1) is a member of the MAPKKK superfamily and has been characterized as a component of the TGF-beta/bone morphogenetic protein signaling pathway. TAK1 function has been extensively studied in cultured cells, but its in vivo function is not fully understood. In this study, we isolated a Drosophila homolog of TAK1 (dTAK1) which contains an extensively conserved NH(2)-terminal kinase domain and a partially conserved COOH-terminal domain. To learn about possible endogenous roles of TAK1 during animal development, we generated transgenic flies which express dTAK1 or the mouse TAK1 (mTAK1) gene in the fly visual system. Ectopic activation of TAK1 signaling leads to a small eye phenotype, and genetic analysis reveals that this phenotype is a result of ectopically induced apoptosis. Genetic and biochemical analyses also indicate that the c-Jun amino-terminal kinase (JNK) signaling pathway is specifically activated by TAK1 signaling. Expression of a dominant negative form of dTAK during embryonic development resulted in various embryonic cuticle defects including dorsal open phenotypes. Our results strongly suggest that in Drosophila melanogaster, TAK1 functions as a MAPKKK in the JNK signaling pathway and participates in such diverse roles as control of cell shape and regulation of apoptosis. |
| Databáze: | OpenAIRE |
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