Exposure temperature, but not donor age, is a confounding factor for in vitro translocation production by chronic irradiation
| Autor: | W A Hsieh, D. P. Morrison, J. N. Lucas, K. L. Gale, Wen Deng, N. Galvan |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male DNA Repair Lymphocyte Alpha (ethology) Chromosomal translocation Biology Translocation Genetic Andrology medicine Humans Radiology Nuclear Medicine and imaging Lymphocytes Irradiation Incubation Aged Genetics Radiological and Ultrasound Technology medicine.diagnostic_test Age Factors Temperature Dose-Response Relationship Radiation Middle Aged In vitro medicine.anatomical_structure Tritium Relative Biological Effectiveness DNA Damage Fluorescence in situ hybridization |
| Zdroj: | International Journal of Radiation Biology. 75:673-680 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/095530099140005 |
| Popis: | To assess the effects of incubation temperature during irradiation, and of donor age, on the in vitro induction of chromosomal translocations in human lymphocytes.Lymphocytes from six human male donors were scored, using fluorescence in situ hybridization, for the presence of chromosomal translocations involving chromosomes 1 to 6 after in vitro, chronic exposure (delivered continuously over 48 h at 37 degrees C or at 20 degrees C) to tritium beta-rays or 60Co gamma-rays.No age-related difference in the alpha coefficients of the fitted induction curves was observed for gamma-ray-exposed lymphocytes obtained from four donors whose ages ranged from 24 to 79 years, or for tritium beta-ray-exposed lymphocytes from two donors aged 36 and 62 years. Duplicate samples from one donor, irradiated concurrently at 20 degrees C or 37 degrees C, gave significantly different alpha coefficients: 0.128+/-0.008 and 0.053+/-0.004, respectively (p0.0001). The S-ratio (the ratio of induced complete to incomplete translocations) was found to be independent of radiation dose, donor age and exposure temperature.For biodosimetry in chronic irradiation situations, the use of alpha coefficients derived from the dose-response curves of cells chronically irradiated in vitro at body temperature is recommended. With respect to induction rates, donor age does not appear to be a confounding factor. The S-ratio is independent of radiation doses, exposure temperatures, or donor ages. |
| Databáze: | OpenAIRE |
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