Expression and Secretion of Neural Cell Adhesion Molecules by Human Pituitary Adenomas
| Autor: | J M Burrin, I De Jong, S J B Aylwin, Y Olabiran, J. F. Geddes, D F Wood, J P Monson |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adenoma
0301 basic medicine Pituitary gland medicine.medical_specialty Cell Adhesion Molecules Neuronal Clinical Biochemistry Immunocytochemistry Biology 03 medical and health sciences Pituitary adenoma Internal medicine medicine Humans Pituitary Neoplasms Secretion 030102 biochemistry & molecular biology Cell adhesion molecule General Medicine medicine.disease Immunohistochemistry Pituitary Hormones 030104 developmental biology medicine.anatomical_structure Endocrinology nervous system Cell culture Cancer research Neural cell adhesion molecule |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 36:660-665 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/000456329903600516 |
| Popis: | Neural cell adhesion molecules (NCAMs) are found predominantly in neural, muscle and endocrine cells. Recent interest has focused on their potential role in tumorigenesis. We have analysed the expression and secretion of NCAM in a series of 48 human pituitary adenomas. Immunocytochemical analysis of 19 adenomas demonstrated NCAM expression in all tumours with, in each case, diffuse cytoplasmic staining being found with variable membrane accentuation. There were no apparent differences in the expression of immunoreactivity seen on sections between individual tumours. Cell culture media from 43 dispersed human pituitary tumours were analysed by immunoassay for the secretion of soluble NCAM and all the pituitary hormones. In contrast to the immunocytochemical studies, soluble NCAM was released from only 27% of human pituitary tumours, but this was not related to tumour type nor was the amount of soluble NCAM released correlated with the amount of pituitary hormone secreted by each adenoma. NCAM expression is common to all human pituitary adenoma types and the observed differences in release of soluble NCAM between individual tumours may reflect different molecular mechanisms, altering adhesive interactions between normal and adenomatous tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|