Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin
| Autor: | Takayuki Kameyama, Shigenori Kanaya, Tadayuki Imanaka, Suthep Thaniyavarn, Mitsuru Haruki, Niran Roongsawang, Jiraporn Thaniyavarn, Masaaki Morikawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Transferases (Other Substituted Phosphate Groups) Bacillus subtilis Microbiology DNA Ribosomal Peptides Cyclic surfactin chemistry.chemical_compound biosurfactants Bacterial Proteins Species Specificity RNA Ribosomal 16S halotolerant Seawater Amino Acid Sequence Cloning Molecular Peptide sequence Soil Microbiology Saline Solution Hypertonic Bacillaceae biology Strain (chemistry) Sequence Homology Amino Acid bacillomycin L Fatty Acids Nucleic acid sequence Lipopeptide General Medicine biology.organism_classification Thailand lipopeptides Carbon Molecular Weight RNA Bacterial plipastatin chemistry Biochemistry Genes Bacterial Fermentation Halotolerance Food Microbiology Molecular Medicine Surfactin Water Microbiology Oligopeptides Sequence Alignment |
| Zdroj: | EXTREMOPHILES. 6(6):499-506 |
| ISSN: | 1431-0651 |
| Popis: | Twenty-three halotolerant and biosurfactant producing strains were collected from salty conditions in central Thailand. One of the strains designated BBK-1 produced the biosurfactants with the highest activity. BBK-1 was isolated from fermented foods and was identified as B. subtilis based on its physiological characteristics and 16S rRNA gene sequence. We show that the strain grows in media containing NaCl up to 16% (w/v) and produces biosurfactants in NaCl up to 8%. We found that B. subtilis BBK-1 produces three kinds of surface-active lipopeptides simultaneously. By their respective molecular weights and amino acid compositions, it is indicated that these lipopeptides are bacillomycin L, plipastatin, and surfactin. In order to analyze the production mechanism of lipopeptides further in the strain, a generally important biosynthetic gene encoding 4'-phosphopantetheinyl transferase was cloned and sequenced. The gene existed in a single copy in the genome and the deduced amino acid sequence was almost identical to that of Lpa-14 from B. subtilis strain RB14, which co-produces iturin A and surfactin. |
| Databáze: | OpenAIRE |
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