3D printed Polylactid Acid based porous scaffold for bone tissue engineering: an in vitro study
| Autor: | Simona Bodnárová, Marek Vojtko, Gabriela Ižaríková, Radovan Hudák, Ľuboš Danišovič, Jozef Živčák, Teodor Tóth, Denisa Harvanová, Jana Plsikova, Ján Rosocha, Sylvia Gromošová |
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| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Biocompatibility Polyesters Biomedical Engineering Biophysics Bioengineering Bone and Bones Biomaterials stomatognathic system Periosteum Cell Adhesion medicine Humans Bovine serum albumin Progenitor cell Cytotoxicity Cells Cultured Cell Proliferation Acid-Base Equilibrium Tissue Engineering Tissue Scaffolds biology Chemistry Stem Cells technology industry and agriculture Middle Aged equipment and supplies In vitro medicine.anatomical_structure Cell culture Printing Three-Dimensional biology.protein Female Tomography X-Ray Computed Porosity Biomedical engineering |
| Zdroj: | Acta of Bioengineering and Biomechanics. 21 |
| ISSN: | 1509-409X |
| Popis: | Purpose The objective of this study was to fabricate PLA-based porous scaffold by 3D printing technology and to evaluate their cytotoxicity and biocompatibility under in vitro conditions in respect to bone tissue engineering. Material and methods Pure PLA in filamentous form was processed via 3D printing technology of fused filament fabrication into porous scaffolds. The structure and porosity of scaffolds were measured by metrotomography. PLA scaffolds were pre-treated by human serum, foetal bovine serum and complete cell culture medium to enhance bio-attractivity of the scaffold's surface for the adherence of the cells. Cells were enzymatically isolated from the periosteum of the proximal tibia and then expanded in monolayer. Periosteum-derived osteoprogenitors (PDOs) were seeded on the pre-treated PLA scaffolds and subsequent cell proliferation was measured by commercially available cell proliferation assay. Adherence of PDOs on the PLA scaffold was confirmed by scanning electron microscopy (SEM). Results Prepared scaffolds had well-defined structure and were characterized by uniform distribution of pores. They were non-toxic and biocompatible with PDOs, however, PLA scaffold with the periosteum-derived progenitor cells was significantly better in the group of scaffolds pre-treated with normal human serum. Conclusions The obtained PLA porous scaffolds favored attachment of periosteum derived progenitors and proliferation, furthermore, cells penetrated into the scaffold through the interstitial pores which was meaningful for cytocompatibility evaluation. |
| Databáze: | OpenAIRE |
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