Effect of Peptide AEDG on Telomere Length and Mitotic Index of PHA-Stimulated Human Blood Lymphocytes
| Autor: | Anna A. Pendina, Anastasiia V. Petrovskaia-Kaminskaia, Olga A. Efimova, Andrei V. Tikhonov, Natalia Linkova, Lubov’ I. Petrova, Mikhail I. Krapivin, V. S. Baranov, Alla S. Koltsova, V. Kh. Khavinson |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Mitotic index Younger age Adolescent Peptide Biology Lymphocyte Activation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Young Adult 0302 clinical medicine Internal medicine medicine Mitotic Index Humans Lymphocytes Phytohemagglutinins Incubation Metaphase In Situ Hybridization Fluorescence chemistry.chemical_classification Human blood General Medicine Middle Aged Telomere Middle age 030104 developmental biology Endocrinology chemistry 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of experimental biology and medicine. 168(1) |
| ISSN: | 1573-8221 |
| Popis: | We studied the effect of peptide AEDG on telomere length and mitotic index of PHA-stimulated blood lymphocytes from young (18-22 years, N=5) and middle-aged (49-54 years, N=6) men. In the younger age group, no significant changes in the mitotic index were detected, while in the middle-aged group, a decrease in this parameter was found in one case. The relative length of telomeric regions of metaphase chromosomes was evaluated by in situ fluorescence hybridization with DNA probes specific to telomeres. After incubation with peptide AEDG, significant changes in the relative telomere length were found in 7 of 11 individuals (3 cases in the younger age group and 4 cases in the middle age group). Significant increase in telomere length after exposure to peptide AEDG was revealed in 5 cases, including two individuals of the younger age group (by 41 and 55%) and three individuals of the middle age group (by 156, 18, and 76%). In one individual of the younger age group and in one of the middle-age group, a significant decrease in telomere length (by 37 and 15%, respectively) was found. A tendency to normalization of telomere lengths was noted: this parameter increased in individuals with initially lower telomere length relative to the group mean value and decreased in individuals with initially longer telomeres compared to the mean length in the group. |
| Databáze: | OpenAIRE |
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