Overexpression of plant ferredoxin-like protein promotes salinity tolerance in rice (Oryza sativa)
| Autor: | Mei-Hsuan Ho, Mang-Jye Ger, Hsien-Yu Chao, Hsiang-En Huang, Hsiang Chang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Salinity
Antioxidant Physiology medicine.medical_treatment Plant Science Biology chemistry.chemical_compound Gene Expression Regulation Plant Botany Genetics medicine Abscisic acid Gene Plant Proteins chemistry.chemical_classification Reactive oxygen species Oryza sativa Abiotic stress fungi food and beverages Oryza Salt Tolerance Plants Genetically Modified Genetically modified rice chemistry Ferredoxins |
| Zdroj: | Plant Physiology and Biochemistry. 155:136-146 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2020.07.025 |
| Popis: | High-salinity stress is one of the major limiting factors on crop productivity. Physiological strategies against high-salinity stress include generation of reactive oxygen species (ROS), induction of stress-related genes expression, accumulation of abscisic acid (ABA) and up-regulation of antiporters. ROS are metabolism by-products and involved in signal transduction pathway. Constitutive expression of plant ferrodoxin-like protein (PFLP) gene enhances pathogen-resistance activities and root-hair growth through promoting ROS generation. However, the function of PFLP in abiotic stress responses is unclear. In this study, PFLP-1 and PFLP-2-transgenic rice plants were generated to elucidate the role of PFLP under salinity stress. PFLP overexpression significantly increased salt tolerance in PFLP-transgenic rice plants compared with non-transgenic plants (Oryza sativa japonica cv. Tainung 67, designated as TNG67). In high-salinity conditions, PFLP-transgenic plants exhibited earlier ROS production, higher antioxidant enzyme activities, higher ABA accumulation, up-regulated expression of stress-related genes (OsRBOHa, Cu/Zn SOD, OsAPX, OsNCED2, OsSOS1, OsCIPK24, OsCBL4, and OsNHX2), and leaf sodium ion content was lower compared with TNG67 plant. In addition, transgenic lines maintained electron transport rates and contained lower malondialdhyde (MDA) content than TNG67 plant did under salt-stress conditions. Overall results indicated salinity tolerance was improved by PFLP overexpression in transgenic rice plant. The PFLP gene is a potential candidate for improving salinity tolerance for valuable agricultural crops. |
| Databáze: | OpenAIRE |
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