Single mRNP Analysis Reveals that Small Cytoplasmic mRNP Granules Represent mRNA Singletons
Autor: | Finn Cilius Nielsen, Àngels Mateu-Regué, Christian Hellriegel, Ole Winther, Frederik Otzen Bagger, Jan Christiansen |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Immunoprecipitation Cytoplasmic Granules Poly(A)-Binding Protein I Ribosome General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Humans MRNA transport RNA Messenger Nuclear pore lcsh:QH301-705.5 Messenger RNA Binding Sites Chemistry RNA-Binding Proteins RNA Translation (biology) Cell biology 030104 developmental biology Microscopy Fluorescence Ribonucleoproteins lcsh:Biology (General) Cytoplasm Nuclear Pore Y-Box-Binding Protein 1 Poly A 030217 neurology & neurosurgery HeLa Cells |
Zdroj: | Mateu-Regué, À, Christiansen, J, Bagger, F O, Winther, O, Hellriegel, C & Nielsen, F C 2019, ' Single mRNP Analysis Reveals that Small Cytoplasmic mRNP Granules Represent mRNA Singletons ', Cell Reports, vol. 29, no. 3, pp. 736-748, e1-e4 . https://doi.org/10.1016/j.celrep.2019.09.018 Cell Reports, Vol 29, Iss 3, Pp 736-748.e4 (2019) |
Popis: | Summary: Small cytoplasmic mRNP granules are implicated in mRNA transport, translational control, and decay. Using super-resolution microscopy and fluorescence correlation spectroscopy, we analyzed the molecular composition and dynamics of single cytoplasmic YBX1_IMP1 mRNP granules in live cells. Granules appeared elongated and branched, with patches of IMP1 and YBX1 distributed along mRNA, reflecting the attachment of the two RNA-binding proteins in cis. Particles form at the nuclear pore and do not associate with translating ribosomes, so the mRNP is a repository for mRNAs awaiting translation. In agreement with the average number of mRNA-binding sites derived from crosslinked immunoprecipitation (CLIP) analyses, individual mRNPs contain 5–15 molecules of YBX1 and IMP1 and a single poly(A) tail identified by PABPC1. Taken together, we conclude that small cytoplasmic mRNP granules are mRNA singletons, thus depicting the cellular transcriptome. Consequently, expression of functionally related mRNAs in RNA regulons is unlikely to result from coordinated assembly. : Using super-resolution microscopy and fluorescence correlation spectroscopy, Mateu-Regué et al. analyze the composition of single cytoplasmic YBX1_IMP1 mRNP granules in live cells. mRNPs are found to represent mRNA singletons depicting the cellular transcriptome. Consequently, mRNA regulons are unlikely to result from coordinated assembly. Keywords: RNA granules, mRNP granules, IMP1, IGF2BP1, YBX1, singleton, structured illumination microscopy, localization microscopy, fluorescence correlation spectroscopy, mRNA regulons |
Databáze: | OpenAIRE |
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