Ferulic acid loaded microspheres reinforced in 3D hybrid scaffold for antimicrobial wound dressing
| Autor: | Satheshkumar Kesavan, Balaraman Madhan, Arun Gopinath, Singaravelu Sivakumar, Ragothaman Murali, Chinnaiyan Senthilkumar, Deepika Arathanaikotti |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Staphylococcus aureus Biocompatibility Coumaric Acids 02 engineering and technology Biochemistry Fibrin Ferulic acid Chitosan 03 medical and health sciences chemistry.chemical_compound Mice Anti-Infective Agents Structural Biology Escherichia coli Animals Molecular Biology 030304 developmental biology 0303 health sciences biology General Medicine Adhesion 021001 nanoscience & nanotechnology Actin cytoskeleton Bandages Microspheres chemistry Chemical engineering biology.protein NIH 3T3 Cells 0210 nano-technology Wound healing |
| Zdroj: | International journal of biological macromolecules. 177 |
| ISSN: | 1879-0003 |
| Popis: | Here we report the preparation of biomimetic fibrin/chitosan/keratin hybrid scaffolds with a synergistic combination of ferulic acid loaded silica microspheres for antimicrobial wound dressing applications. The infrared and X-ray powder diffraction studies confirm the homogenous nature of the prepared hybrid scaffolds without any major interactions between the constituents. The developed hybrid scaffolds show good thermal, porosity, compression and water uptake properties. Scanning electron microscopic analysis shows that the as-synthesized ferulic acid loaded silica microspheres exhibit an average size of 35 ± 10 μm and also exposes the smooth surface with interconnected porosity in the prepared hybrid scaffolds. The incorporated ferulic acid loaded silica microspheres hybrid scaffolds show effective antimicrobial activity against the common wound pathogens. In vitro NIH3T3 fibroblast cell culture and drug release studies reveal that the prepared hybrid scaffolds have enhanced cell proliferation and adhesion with a prolonged drug release for about 72 h. In vitro wound healing and actin cytoskeleton analysis reveal that the incorporated ferulic acid loaded silica microspheres in fibrin/chitosan/keratin hybrid scaffolds facilitates cell growth and migration to damaged area through cell-cell interactions. These results suggest that the prepared hybrid scaffolds with ferulic acid loaded silica microspheres have great potential for soft tissue engineering applications particularly for the treatment of chronic and infected wounds. |
| Databáze: | OpenAIRE |
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