LncRNA PTPRG-AS1 Promotes the Metastasis of Hepatocellular Carcinoma by Enhancing YWHAG
| Autor: | Yanqing Liu, Zhi-Sheng Zhang, Lu Wang, Gao-Yang Chen, Yan Li |
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| Rok vydání: | 2021 |
| Předmět: |
Reporter gene
Article Subject business.industry Competing endogenous RNA Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease medicine.disease_cause digestive system diseases Metastasis Real-time polymerase chain reaction Oncology Downregulation and upregulation Hepatocellular carcinoma Cancer research Medicine YWHAG business Carcinogenesis RC254-282 Research Article |
| Zdroj: | Journal of Oncology Journal of Oncology, Vol 2021 (2021) |
| ISSN: | 1687-8450 |
| Popis: | Objectives. Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. LncRNA PTPRG-AS1 (PTPRG-AS1) has been confirmed to function as a regulator in various cancers, whose function during HCC tumorigenesis is still not clear now. Thus, we aim to dig out the biological function and its mechanisms of PTPRG-AS1 in HCC. Methods. PTPRG-AS1 relative expression in tissues and cells was detected and analyzed using real-time quantitative PCR (qRT-PCR). Subcellular distribution of PTPRG-AS1 was examined by FISH experiments. The effects of PTPRG-AS1 in the growth of HCC were studied by in vitro CCK-8 experiments, transwell invasion experiments, and in vivo xenograft tumor experiments. Dual-Luciferase reporter assay was performed to verify the interaction between PTPRG-AS1 and miR-199a-3p or miR-199a-3p and its target gene, YWHAG. Results. PTPRG-AS1 was upregulated in HCC tissues compared with adjacent normal tissues. We identified PTPRG-AS1 mainly localized in the cytoplasm of HCC cells. Downregulation of PTPRG-AS1 suppressed HCC progression, while overexpression of PTPRG-AS1 showed the opposite effects. Furthermore, PTPRG-AS1 served as a miR-199a-3p sponge and positively regulated YWHAG expression. Besides, PTPRG-AS1 could promote HCC through miR-199a-3p/YWHAG axis. Conclusions. Taken together, we demonstrated PTPRG-AS1 may serve as a ceRNA and reversely regulates the expression of miR-199a-3p, thus facilitating HCC tumorigenesis and metastasis, which is expected to provide new clues for the treatment of HCC. |
| Databáze: | OpenAIRE |
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