Surface-modified magnetite nanoparticles act as aneugen-like spindle poison
| Autor: | Andrea Babelova, Filip Rázga, Alena Gábelová, Monika Mesárošová, Monika Ursinyova, Barbora Buliaková, Michal Šelc, Veronika Némethová, Lívia Sebová, Ivan Chalupa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Context (language use) Antineoplastic Agents Spindle Apparatus 03 medical and health sciences chemistry.chemical_compound Tubulin medicine Humans General Materials Science Magnetite Nanoparticles Micronuclei Chromosome-Defective A549 cell biology Aneugens Molecular biology 3. Good health Spindle apparatus Spindle poison 030104 developmental biology Nanomedicine chemistry Centrosome A549 Cells biology.protein Biophysics Molecular Medicine Iron oxide nanoparticles medicine.drug DNA Damage |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine |
| ISSN: | 1549-9642 |
| Popis: | Iron oxide nanoparticles are one of the most promising types of nanoparticles for biomedical applications, primarily in the context of nanomedicine-based diagnostics and therapy; hence, great attention should be paid to their bio-safety. Here, we investigate the ability of surface-modified magnetite nanoparticles (MNPs) to produce chromosome damage in human alveolar A549 cells. Compared to control cells, all the applied MNPs increased the level of micronuclei moderately but did not cause structural chromosomal aberrations in exposed cells. A rise in endoreplication, polyploid and multinuclear cells along with disruption of tubulin filaments, downregulation of Aurora protein kinases and p53 protein activation indicated the capacity of these MNPs to impair the chromosomal passenger complex and/or centrosome maturation. We suppose that surface-modified MNPs may act as aneugen-like spindle poisons via interference with tubulin polymerization. Further studies on experimental animals revealing mechanisms of therapeutic-aimed MNPs are required to confirm their suitability as potential anti-cancer drugs. |
| Databáze: | OpenAIRE |
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