Quality assurance for antimicrobial susceptibility testing of Neisseria gonorrhoeae in Latin American and Caribbean countries, 2013-2015
| Autor: | Aura Helena Corredor, Irene Martin, Pam Sawatzky, Jo-Anne R. Dillon, Maria Elena Trigoso Carvallo, Mario Fabian Martinez, Alina Llop Hernandez, Patricia Galarza, Olga Marina Sanabria Cruz, José E Moreno, Pamela Araya Rodriguez, Graciela Borthagaray, S D Thakur, Daisy Payares, Marina Chiappe |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Quality Control Veterinary medicine Spectinomycin 030106 microbiology Dermatology medicine.disease_cause Ciprofloxacin/pharmacology Agar dilution Anti-Bacterial Agents/pharmacology 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Azithromycin/pharmacology medicine Humans 030212 general & internal medicine antimicrobial resistance gonorrhoea Laboratories Hospital/statistics & numerical data Microbial Sensitivity Tests/methods/standards Etest Neisseria gonorrhoeae/drug effects/isolation & purification Ceftriaxone/pharmacology Gonorrhea/drug therapy/epidemiology/microbiology business.industry Latin America/epidemiology Reproducibility of Results purl.org/pe-repo/ocde/ford#3.02.15 [https] Antimicrobial Penicillin Caribbean Region/epidemiology Infectious Diseases Latin America Laboratory Proficiency Testing/methods/standards Epidemiological Monitoring Neisseria gonorrhoeae surveillance business Cefixime Disk Diffusion Antimicrobial Tests/standards medicine.drug |
| Popis: | ObjectivesA Neisseria gonorrhoeae antimicrobial susceptibility quality control comparison programme was re-established in Latin America and the Caribbean to ensure antimicrobial susceptibility data produced from the region are comparable nationally and internationally.MethodsThree panels, consisting of N. gonorrhoeae isolates comprising reference strains and other characterised isolates were sent to 11 participating laboratories between 2013 and 2015. Antimicrobial susceptibilities for these isolates were determined using agar dilution, Etest or disc diffusion methods. Modal minimum inhibitory concentrations (MICs) for each panel isolate/antibiotic combination were calculated. The guidelines of the Clinical and Laboratory Standards Institute were used for interpretations of antimicrobial susceptibility. The agreement of MICs with the modal MICs was determined for each of the participating laboratories as well as for each of the antibiotics tested.ResultsFive of 11 laboratories that participated in at least one panel had an overall average agreement between participants’ MIC results and modal MICs of >90%. For other laboratories, agreements ranged from 60.0% to 82.4%. The proportion of agreement between interpretations for all the antibiotics, except penicillin and tetracycline, was >90%. The percentages of agreement between MIC results and their modes for erythromycin, spectinomycin, cefixime and azithromycin were >90%. Tetracycline, ceftriaxone and ciprofloxacin agreement ranged from 84.5% to 89.1%, while penicillin had 78.8% agreement between MICs and modal MICs.ConclusionsThe participating laboratories had acceptable results, similar to other international quality assurance programmes. It is important to ensure continuation of the International Gonococcal Antimicrobial Susceptibility Quality Control Comparison Programme to ensure that participants can identify and correct any problems in antimicrobial susceptibility testing for N. gonorrhoeae as they arise and continue to generate reproducible and reliable data. |
| Databáze: | OpenAIRE |
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