An enterovirus strain isolated from diabetic child belongs to a genetic subcluster of echovirus 11, but is also neutralised with monotypic antisera to coxsackievirus A9
| Autor: | Alexander N. Lukashev, Kaija Salmela, Mervi Eskelinen, Anja Paananen, Merja Roivainen, Petri Ylipaasto, Shubhada Bobegamage, Tapani Hovi, Haider Al-Hello |
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| Rok vydání: | 2008 |
| Předmět: |
Echovirus
Molecular Sequence Data Echovirus Infections Coxsackievirus medicine.disease_cause Antibodies Viral Peptide Mapping Virus Neutralization Uveitis 03 medical and health sciences Islets of Langerhans Neutralization Tests Virology medicine Enterovirus Infections Animals Humans Amino Acid Sequence Serotyping Child Antigens Viral Cells Cultured 030304 developmental biology Antiserum 0303 health sciences biology Phylogenetic tree 030306 microbiology Sequence Analysis DNA biology.organism_classification 3. Good health Enterovirus B Human Diabetes Mellitus Type 1 Capsid Enterovirus Peptides |
| Zdroj: | The Journal of general virology. 89(Pt 8) |
| ISSN: | 0022-1317 |
| Popis: | An enterovirus strain (designated D207) isolated from a Slovakian diabetic child and originally serotyped as coxsackievirus A9 (CAV-9) was found to cause rapid cytolysis coinciding with severe functional damage of the surviving cells in primary cultures of human pancreatic islets. This finding prompted us to clone the isolate for full-length genome sequencing and molecular characterization as the prototype strain of CAV-9 is known to cause only minimal damage to insulin-producing β-cells. Based on capsid-coding sequence comparisons, the isolate turned out to be echovirus 11 (E-11). Phylogenetic analyses demonstrated that E-11/D207 was closely related to a specific subgroup B of E-11 strains known to cause uveitis. To study further antigenic properties of isolate E-11/D207 and uveitis-causing E-11 strains, neutralization experiments were carried out with CAV-9- and E-11-specific antisera. Unlike the prototype strains, the isolate E-11/D207 and uveitis-causing E-11 strains were well neutralized with both CAV-9- and E-11-specific antisera. Attempts to identify recombination of the capsid coding sequences as a reason for double-reactivity using the Simplot analysis failed to reveal major transferred motifs. However, peptide scanning technique was able to identify antigenic regions of capsid proteins of E-11/D207 as well as regions cross-reacting with an antiserum raised to CAV-9. Thus, double specificity of E-11/D207 seems to be a real characteristic shared by the phylogenetically closely related virus strains in the genetic subgroup B of E-11. |
| Databáze: | OpenAIRE |
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