A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1)
| Autor: | Stephen N. Jones, Anna M. Cerny, Nehal Mohamed, Robert W. Finberg, Anne Nicholson-Weller, Leslie S. Casey, Steven M. Jones, Alexander Repik, Steve E. Pincus, Lloyd B. Klickstein, Damon R. Asher, George L. Spitalny, Ionita Ghiran |
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| Rok vydání: | 2005 |
| Předmět: |
Genetically modified mouse
Erythrocytes Complement receptor 1 Immunology Dose-Response Relationship Immunologic Mice Transgenic Complement receptor Antigen-Antibody Complex Virus Mice Antigen In vivo Antibodies Bispecific Blood-Borne Pathogens Immunology and Allergy Animals Humans biology Bispecific monoclonal antibody Molecular biology Complement system Receptors Complement Mice Inbred C57BL Disease Models Animal Microscopy Fluorescence Animal Studies biology.gene Bacteriophage phi X 174 Papio |
| Zdroj: | Clinical and experimental immunology. 140(2) |
| ISSN: | 0009-9104 |
| Popis: | Summary Complement receptor 1 (CR1) on the surface of human erythrocytes facilitates intravascular clearance of complement-opsonized pathogens. The need for complement activation can be circumvented by directly coupling the organism to CR1 using a bispecific monoclonal antibody heteropolymer (HP). Lack of a functional homologue to CR1 on mouse erythrocytes has made it difficult to study HP-dependent clearance of pathogens in small animals. We have developed a transgenic mouse that expresses human CR1 on erythrocytes. CR1 antigen is of appropriate size and in a clustered distribution as confirmed by immunoblotting and fluorescence microscopy, respectively. HP that immobilized bacteriophage ΦX174 prototype pathogen to erythrocyte CR1 of the transgenic mice increased the rate of clearance of the virus compared with HP that bound bacteriophage, but not CR1. This transgenic mouse model will allow evaluation of different HPs for their in vivo efficacy and potential as human therapeutics. |
| Databáze: | OpenAIRE |
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