Lac Repressor Binding to DNA not containing the Lac Operator and to Synthetic Poly dAT
| Autor: | Arthur D. Riggs, Syr-Yaung Lin |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
DNA
Bacterial Male Operator (biology) Clostridium perfringens Stereochemistry Polynucleotides lac operon Repressor Lactose Lac repressor medicine.disease_cause chemistry.chemical_compound Genes Regulator Operon medicine Animals Escherichia coli Multidisciplinary Chemistry Osmolar Concentration Phosphorus Isotopes DNA Spermatozoa Dissociation constant bacteria Salmonidae |
| Zdroj: | Nature. 228:1184-1186 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/2281184a0 |
| Popis: | THE lac represser binds very specifically and tightly to the lac operator1–3. In conditions where the repressor binds to operator with a dissociation constant of 10−13 M, the binding of represser to DNA not containing the Escherichia coli lac operator (non-operator DNA) was less tight by several orders of magnitude3. Although weak, we thought this non-operator DNA binding could be highly significant and deserved careful study. We report here on the binding of lac represser to non-operator DNA from several natural sources and also to synthetic poly dAT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|