The role of the gap junction protein connexin43 in B lymphocyte motility and migration
| Autor: | Farnaz Pournia, Kate Choi, May Dang-Lawson, Letitia Falk, Linda Matsuuchi, Steven Machtaler, Christian C. Naus |
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| Rok vydání: | 2013 |
| Předmět: |
Cell type
Biophysics Biology Biochemistry B-lymphocyte Mice Structural Biology Cell Line Tumor Genetics medicine Animals Cytoskeleton Cell adhesion Molecular Biology B cell B-Lymphocytes Gap junction Transendothelial and Transepithelial Migration rap1 GTP-Binding Proteins Cell Biology Actin cytoskeleton Chemokine CXCL12 Cx43 Cell biology Rats Actin Cytoskeleton Protein Transport medicine.anatomical_structure Connexin 43 cardiovascular system Rap1 sense organs Intracellular |
| Zdroj: | FEBS letters. 588(8) |
| ISSN: | 1873-3468 |
| Popis: | The gap junction family of proteins is widely expressed in mammalian cells and form intercellular channels between adjacent cells, as well as hemichannels, for transport of molecules between the cell and the surrounding environment. In addition, gap junction proteins have recently been implicated as important for the regulation of cell adhesion and migration in a variety of cell types. The gap junction protein connexin43 (Cx43) regulates B lymphocyte adhesion, BCR- and LFA-1-mediated activation of the GTPase Rap1, and cytoskeletal rearrangements resulting in changes to cell shape and membrane spreading. We demonstrate here that the actin cytoskeleton is important for the distribution of Cx43 in the B cell plasma membrane and for other cell processes involving the cytoskeleton. Using shRNA knockdown of Cx43 in B lymphoma cells we show that Cx43 is also necessary for chemokine-mediated Rap 1 activation, motility, CXCL12-directed migration, and movement across an endothelial cell monolayer. These results demonstrate that in addition to its role in B cell spreading, Cx43 is an important regulator of B-cell motility and migration, processes essential for normal B-cell development and immune responses. |
| Databáze: | OpenAIRE |
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