UNBS5162 inhibits colon cancer growth via suppression of PI3K/Akt signaling pathway
| Autor: | Cun-Chuan Wang, Fan Zhang, Huizeng Lv, Xiaoyong Ye, Jiming Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Colorectal cancer Down-Regulation Antineoplastic Agents Apoptosis General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Phosphatidylinositol 3-Kinases 0302 clinical medicine Cell Movement medicine Humans Urea Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Chemistry Akt/PKB signaling pathway Cell growth General Medicine medicine.disease HCT116 Cells Naphthalimides 030104 developmental biology Tumor progression 030220 oncology & carcinogenesis Colonic Neoplasms Cancer research Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Medecine sciences : M/S. |
| ISSN: | 1958-5381 |
| Popis: | Colon cancer is a common cause of cancer-related death worldwide. However, the underlying mechanism of tumor progression of colon cancer remains far from being elucidated. In the present study, we report the role of UNBS5162 in colon cancer. UNBS5162 is a naphthalimide that can intercalate into DNA and suppress the expression level of CXCL chemokines. Here, we investigated its effect on cell proliferation, mobility and apoptosis in HCT116 cells, and explored the underlying mechanism. A CCK8 assay revealed that UNBS5162 can block the proliferation of colon cancer cells. Base on a Transwell assay, we showed that cell migration and invasion ability of HCT116 cells are inhibited by UNBS5162. In addition, Annexin V-FITC/PI assay and Western blot analysis were performed to detect whether UNBS5162 could induce cell apoptosis. The results indicated that UNBS5162 increases the number of apoptotic cells remarkably. Furthermore, Western blot analysis demonstrated that UNBS5162 down-regulates the expression level of Bcl2, and up-regulates that of Bax as well as the level of activated Caspase-3. Moreover, we examined the impact of UNBS5162 on PI3K/Akt signaling pathway. UNBS5162 substantially inhibited the phosphorylation of Akt and its downstream effector mTOR, and reduced the expression of p-70. Taken together, these results suggest that UNBS5162 should be considered as a potent therapeutic anticancer agent that targets the PI3K/AKT signaling pathway. |
| Databáze: | OpenAIRE |
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