Nanomechanische Eigenschaften von Keratin 8/18 - von Einzelfilamenten zu Netzwerken

Autor:	Leitner, Anke
Jazyk:	němčina
Rok vydání:	2012
Předmět:	Schermodul DDC 530 / Physics Keratin Persistenlänge ddc:530 Schubmodul macromolecular substances Assemblykinetik Zellskelett Zytoskelett
Popis:	The mechanical properties of epithelial cells are mainly determined by the cytoskeleton. The cytoskeleton consists of three different protein networks: microtubules, actin filaments and intermediate filaments. In pancreatic cancer cells especially the intermediate filament protein keratin provides the stiffness and response to mechanical stimuli. In order to find out more about its mechanical properties it is useful to have a look on in vitro assembled keratin filaments. In the work presented here we investigated the assembly kinetics, the persistence length and the mechanical properties of in vitro assembled keratin 8/18 networks in different polymerisation conditions. For this purpose we use microrheology measurements with embedded tracer beads. From electron microscopy images we calculated the meshsize and connectivity of the different network structures and link these results to the mechanical properties of the different networks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad1ac9a8cc678c5ddb8d46e6d1c38827 Zobrazit plný text záznamu