Effect of glutamine on the degradation of glutamine synthetase in hepatoma tissue-culture cells
| Autor: | G M Tomkins, R B Crook |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
chemistry.chemical_classification
Chemistry Immunoprecipitation Glutamine Cell Biology Biochemistry Molecular biology Cell Line Kinetics Tissue culture Liver Neoplasms Experimental Enzyme Glutamate-Ammonia Ligase Glutamine synthetase Dactinomycin Extracellular Animals Specific activity Cycloheximide Molecular Biology Function (biology) Research Article |
| Zdroj: | Biochemical Journal. 176:47-52 |
| ISSN: | 0264-6021 |
| DOI: | 10.1042/bj1760047 |
| Popis: | In certain lines of hepatoma tissue-culture cells, the extracellular glutamine concentration regulates the specific activity of glutamine synthetase. By quantifying the radioactivity in immunoprecipitated glutamine synthetase on polyacrylamide gels, we found that the rate of degradation, but not of synthesis, of glutamine synthetase is a sensitive function of extracellular glutamine. The activiy that degrades this enzyme appears to be labile. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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