Transcriptional analysis of rolling circle replicating plasmid pVT736-1: evidence for replication control by antisense RNA
| Autor: | D J Leblanc, Dominique M. Galli |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA Replication
Transcription Genetic Molecular Sequence Data Biology Aggregatibacter actinomycetemcomitans Microbiology Primer extension RNA Complementary Open Reading Frames Plasmid Bacterial Proteins Peptide Initiation Factors RNA Antisense Promoter Regions Genetic Molecular Biology Genetics Binding Sites Base Sequence Models Genetic DNA Helicases DNA replication Nucleic acid sequence RNA Promoter Gene Expression Regulation Bacterial Molecular biology Antisense RNA DNA-Binding Proteins RNA Bacterial Rolling circle replication Trans-Activators Nucleic Acid Conformation Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 177:4474-4480 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Several plasmids have been described in Actinobacillus actinomycetemcomitans, a gram-negative coccobacillus. Recently, the nucleotide sequence of pVT736-1, a cryptic plasmid of A. actinomycetemcomitans VT736, was determined. This plasmid possesses all the features necessary for rolling circle replication. The present study involved a transcriptional analysis of pVT736-1. Results of Northern (RNA) blot analyses and primer extension studies indicated that the two open reading frames identified in pVT736-1 are each preceded by at least one promoter. Expression of these promoters varied with growth phase. In addition, an antisense RNA (Cop RNA) appeared to control the synthesis of the putative replication protein. To our knowledge, this is the first rolling circle replicating plasmid isolated from a gram-negative organism that has been subjected to such detailed analysis. |
| Databáze: | OpenAIRE |
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