Gene regulatory network and its constituent transcription factors that control nitrogen‐deficiency responses in rice
Autor: | Mitsue Miyao, Taro Kadowaki, Namie Ohtsuki, Ayumi Tezuka, Yonghyun Kim, Yoshiaki Ueda, Koji Kadota, Atsushi J. Nagano, Shuichi Yanagisawa |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Nitrogen Physiology Systems biology Gene regulatory network Plant Science Biology 01 natural sciences 03 medical and health sciences Genome editing Gene Expression Regulation Plant Gene Regulatory Networks Gene Transcription factor Plant Proteins Genetics Oryza sativa Nitrogen deficiency Gene Expression Profiling food and beverages Oryza 030104 developmental biology Function (biology) Transcription Factors 010606 plant biology & botany |
Zdroj: | New Phytologist. 227:1434-1452 |
ISSN: | 1469-8137 0028-646X |
Popis: | Increase in the nitrogen (N)-use efficiency and optimization of N response in crop species are urgently needed. Although transcription factor-based genetic engineering is a promising approach for achieving these goals, transcription factors that play key roles in the response to N deficiency have not been studied extensively. Here, we performed RNA-seq analysis of root samples of 20 Asian rice (Oryza sativa) accessions with differential nutrient uptake. Data obtained from plants exposed to N-replete and N-deficient conditions were subjected to coexpression analysis and machine learning-based pathway inference to dissect the gene regulatory network required for the response to N deficiency. Four transcription factors, including members of the G2-like and bZIP families, were predicted to function as key regulators of gene transcription within the network in response to N deficiency. Cotransfection assays validated inferred novel regulatory pathways, and further analyses using genome-edited knockout lines suggested that these transcription factors are important for N-deficiency responses in planta. Many of the N deficiency-responsive genes, including those encoding key regulators within the network, were coordinately regulated by transcription factors belonging to different families. Transcription factors identified in this study could be valuable for the modification of N response and metabolism. |
Databáze: | OpenAIRE |
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