Adipose-derived stem cells on the healing of ischemic colitis: a therapeutic effect by angiogenesis
| Autor: | Jong Tae Kim, Ki Beom Bae, Hye Jung Jo, Tae Doo Jung, Kwan Hee Hong, Jeong Kim, Young Il Yang, Sun-Hee Kim, Min Sung Ahn, Hyun Ho Joo |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Ileus Angiogenesis Adipose tissue Neovascularization Physiologic Cell Separation Gastroenterology Ischemic colitis Neovascularization Rats Sprague-Dawley Internal medicine Medicine Animals Humans Colitis Fibrin glue Cells Cultured Wound Healing business.industry Stem Cells Cell Differentiation medicine.disease Rats Adipose Tissue Immunology Microvessels medicine.symptom business Wound healing Colitis Ischemic Stem Cell Transplantation |
| Zdroj: | International journal of colorectal disease. 27(11) |
| ISSN: | 1432-1262 |
| Popis: | There has been no specific treatment for ischemic colitis. We verified the effects of adipose-derived stem cells (ASCs) on ischemia-induced colitis in a rat model. Forty male Sprague–Dawley rats (10 weeks old; weight, 350 ± 20 g) were divided into two groups: a control group (only fibrinogen and thrombin injected, n = 20) and an ASC group (local implantation of ASCs mixed with thrombin and fibrinogen, n = 20). An ischemic colitis model was established by modifying Nagahata's methods with double-blind randomization. ASCs (1 × 106 cells) were implanted intramurally into the ischemic area using a fibrin glue mixture. The severity of adhesion, degree of ileus, the number and size of the ulcers, Wallace macroscopic and microscopic scores, and microvascular density were measured. The degree of ileus was significantly lower, and significantly fewer and smaller ulcerations were found in the ASC group than those in the control group. Wallace macroscopic and microscopic scores were lower in the ASC group than in the control group (1.90 ± 1.22 versus 3.25 ± 1.83, p |
| Databáze: | OpenAIRE |
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