Performance of Commercial Mycoplasma hyopneumoniae Serum Enzyme-Linked Immunosorbent Assays under Experimental and Field Conditions
Autor: | Aric McDaniel, Franco Matias Ferreyra, Luis G. Giménez-Lirola, Ronaldo Magtoto, Jeffrey J. Zimmerman, Precy D. Magtoto, Maria M. Merodio, Igor Renan Honorato Gatto, Rachel J. Derscheid, David H. Baum, Maria J. Clavijo, Bailey Arruda, Ana Paula Serafini Poeta Silva, Henrique Meiroz de Souza Almeida |
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Přispěvatelé: | Iowa State University, Universidade Estadual Paulista (UNESP), Pampanga State Agricultural University, PIC North America |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Microbiology (medical) Mycoplasma hyorhinis cross-reactivity 040301 veterinary sciences Swine Enzyme-Linked Immunosorbent Assay Biology Mycoplasma flocculare medicine.disease_cause Cross-reactivity Serum enzymes Microbiology 0403 veterinary science 03 medical and health sciences Mycoplasma Mycoplasma hyopneumoniae medicine Mycoplasma hyosynoviae Animals Oral fluids Immunoassays test performance Pathogen Swine Diseases 04 agricultural and veterinary sciences Pneumonia of Swine Mycoplasmal biology.organism_classification Antibodies Bacterial oral fluids Misclassification error rate misclassification error rate 030104 developmental biology Test performance ELISA Field conditions |
Zdroj: | Journal of Clinical Microbiology Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
ISSN: | 1098-660X |
Popis: | Mycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Mycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Weekly serum and daily oral fluid samples were collected through 56 days postinoculation (dpi). The true status of pigs was established by PCR testing on oral fluids samples over the course of the observation period. Analysis of ELISA performance at various cutoffs found that the manufacturers’ recommended cutoffs were diagnostically specific, i.e., produced no false positives, with the exceptions of 2 ELISAs. An analysis based on overall misclassification error rates found that 4 ELISAs performed similarly, although one assay produced more false positives. In study 2, the 3 best-performing ELISAs from study 1 were compared using serum samples generated under field conditions. Ten 8-week-old pigs were intratracheally inoculated with M. hyopneumoniae. Matched serum and tracheal samples (to establish the true pig M. hyopneumoniae status) were collected at 7- to 14-day intervals through 98 dpi. Analyses of sensitivity and specificity showed similar performance among these 3 ELISAs. Overall, this study provides an assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance. |
Databáze: | OpenAIRE |
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