Positive selection of T-lymphocytes induced by intrathymic injection of a thymic epithelial cell line

A) bone marrow chimaeras, T cells restricted to the MHC-A haplotype are positively selected, whereas MHC-B-reactive thymocytes are not. We investigated whether the introduction of particular thymic stromal elements bearing MHC-B molecules could alter the fate of B-reactive T cells in these (A x B-->A) chimaeras. Thymic epithelial cell (TEC) lines expressing H-2b were introduced by intrathymic injection into (H-2b/s-->H2s) bone marrow chimaeras and we measured their ability to generate H-2b-restricted cytotoxic T-lymphocytes (CTLs). We report here that one TEC line, 427.1, was able positively to select CTLs specific for influenza and vesicular stomatitis virus antigens in association with class I H-2b molecules. In addition, line 427.1 can process cytoplasmic proteins for presentation to H-2Kb- and H-2Db-restricted CTLs. Thus, a TEC line capable of normal class I MHC antigen processing and presentation in vitro can induce positive selection after intrathymic injection. -->

ISSN:	1476-4687 0028-0836
DOI:	10.1038/359729a0
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad093fdae9ad76f34d068c0331d8c2d5 https://doi.org/10.1038/359729a0
Rights:	OPEN
Přírůstkové číslo:	edsair.doi.dedup.....ad093fdae9ad76f34d068c0331d8c2d5
Autor:	Barbara B. Knowles, Michael J. Bevan, Susan J. Faas, Andres G. Grandea, Stanislav Vukmanović
Rok vydání:	1992
Předmět:	Cytotoxicity Immunologic Stromal cell Molecular Sequence Data Antigen presentation Mice Transgenic Thymus Gland Major histocompatibility complex Article Vesicular stomatitis Indiana virus Mice Antigen Tumor Cells Cultured medicine Animals Cytotoxic T cell Antigens Viral Immunity Cellular Multidisciplinary Base Sequence biology H-2 Antigens T lymphocyte Orthomyxoviridae In vitro Cell biology medicine.anatomical_structure Oligodeoxyribonucleotides Radiation Chimera Immunology biology.protein Bone marrow T-Lymphocytes Cytotoxic
Zdroj:	Nature. 359:729-732
ISSN:	1476-4687 0028-0836
DOI:	10.1038/359729a0
Popis:	T lymphocytes recognize antigens as peptide fragments associated with molecules encoded by the major histocompatibility complex (MHC) and expressed on the surface of antigen-presenting cells. In the thymus, T cells bearing alpha beta receptors that react with the MHC molecules expressed by radioresistant stromal elements are positively selected for maturation. In (A x B-->A) bone marrow chimaeras, T cells restricted to the MHC-A haplotype are positively selected, whereas MHC-B-reactive thymocytes are not. We investigated whether the introduction of particular thymic stromal elements bearing MHC-B molecules could alter the fate of B-reactive T cells in these (A x B-->A) chimaeras. Thymic epithelial cell (TEC) lines expressing H-2b were introduced by intrathymic injection into (H-2b/s-->H2s) bone marrow chimaeras and we measured their ability to generate H-2b-restricted cytotoxic T-lymphocytes (CTLs). We report here that one TEC line, 427.1, was able positively to select CTLs specific for influenza and vesicular stomatitis virus antigens in association with class I H-2b molecules. In addition, line 427.1 can process cytoplasmic proteins for presentation to H-2Kb- and H-2Db-restricted CTLs. Thus, a TEC line capable of normal class I MHC antigen processing and presentation in vitro can induce positive selection after intrathymic injection.
Databáze:	OpenAIRE
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