A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri
| Autor: | Stefan Graupner, Wilfried Wackernagel |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Genetics Microbial Genetic Vectors Gene Expression Bioengineering Polymerase Chain Reaction Microbiology Plasmid Bacterial Proteins Pseudomonas Gene expression Drosophila Proteins Promoter Regions Genetic Molecular Biology Gene Serratia marcescens DNA Primers Peptidylprolyl isomerase Expression vector biology Base Sequence Peptidylprolyl Isomerase biology.organism_classification beta-Galactosidase Pseudomonas stutzeri NIMA-Interacting Peptidylprolyl Isomerase Transformation (genetics) Genes Bacterial Insect Proteins Transformation Bacterial Carbohydrate Epimerases Biotechnology |
| Zdroj: | Biomolecular engineering. 17(1) |
| ISSN: | 1389-0344 |
| Popis: | A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq can be quantitated with a separate test plasmid in the strain to be studied. The dod gene of Serratia marcescens was expressed in Pseudomonas stutzeri and was shown to code for D-ribulose-5-phosphate 3-epimerase. |
| Databáze: | OpenAIRE |
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