VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF NORTRIPTYLINE AND PREGABALIN IN BULK AND COMBINED DOSAGE FORMULATIONS
| Autor: | Sunandamma Yeturu, Sreenivasa Rao Battula, Haritha Potluri |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
validation
Active ingredient Chromatography 010401 analytical chemistry Pregabalin General Chemistry nortriptyline 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry stability indicating Stability indicating medicine pregabalin Perchloric acid Nortriptyline HPLC Uv detection Quantitative analysis (chemistry) 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of the Chilean Chemical Society, Volume: 62, Issue: 2, Pages: 3490-3495, Published: JUN 2017 Journal of the Chilean Chemical Society v.62 n.2 2017 SciELO Chile CONICYT Chile instacron:CONICYT |
| ISSN: | 0717-9707 |
| DOI: | 10.4067/s0717-97072017000200013 |
| Popis: | A simple and precise stability indicating RP-HPLC method was developed for simultaneous analysis of drug nortriptyline and pregabalin using BDS (250mm x 4.6 mm, 5μ) C18 column at 210 nm of UV detection. Perchloric acid (0.1%) and acetonitrile in the ratio of 55:45 was used as the mobile phase with a flow rate of 1.0 ml/min and linearity response was established over the concentration range of 5-30 μg/ml for nortriptyline and 37.5-225 μg/ml for pregabalin. The active pharmaceutical ingredients recovered for nortriptyline and pregabalin are in the range of 100.60-101.65% and 100.59-101.74% respectively. The method was validated and was found to be stability indicating and can be successfully utilized for the quantitative analysis of pharmaceutical tablet dosage formulations containing nortriptyline and pregabalin. |
| Databáze: | OpenAIRE |
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