Local injections of adipose-derived mesenchymal stem cells modulate inflammation and increase angiogenesis ameliorating the dystrophic phenotype in dystrophin-deficient skeletal muscle
| Autor: | Rui Curi, Kaio Fernando Vitzel, Maria Tereza Nunes, Renato Tadeu Nachbar, Alcione Lescano de Souza, Jean César Farias de Queiroz, Luís G O de Sousa, Lucas Guimarães-Ferreira, Carlos Hermano da Justa Pinheiro |
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| Rok vydání: | 2011 |
| Předmět: |
Muscle tissue
Male Vascular Endothelial Growth Factor A Cancer Research medicine.medical_specialty Adipose tissue Neovascularization Physiologic Inflammation Biology Mesenchymal Stem Cell Transplantation Injections Dystrophin Transforming Growth Factor beta1 Mice Internal medicine medicine Animals Muscular dystrophy Muscle Skeletal Myogenin Macrophages Mesenchymal stem cell Skeletal muscle Mesenchymal Stem Cells Cell Biology Muscular Dystrophy Animal medicine.disease Transplantation FISIOLOGIA Mice Inbred C57BL Endocrinology medicine.anatomical_structure Phenotype Adipose Tissue Immunology Cytokines medicine.symptom Inflammation Mediators Reactive Oxygen Species Biomarkers |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2629-3277 |
| Popis: | The effects of adipose-derived mesenchymal stem cells (ADMSC) transplantation on degeneration, regeneration and skeletal muscle function were investigated in dystrophin-deficient mice (24-week-old). ADMSC transplantation improved muscle strength and, resistance to fatigue. An increase in fiber cross-sectional area and in the number of fibers with centralized nuclei and augment of myogenin content were observed. In ADMSC-treated muscles a decrease in muscle content of TNF-α, IL-6 and oxidative stress measured by Amplex(®) reagent were observed. The level of TGF-β1 was lowered whereas that of VEGF, IL-10 and IL-4 were increased by ADMSC treatment. An increase in markers of macrophage M1 (CD11 and F4-80) and a decrease in T lymphocyte marker (CD3) and arginase-1 were also observed in ADMSCs-treated dystrophic muscle. No change was observed in iNOS expression. Increased phosphorylation of Akt, p70S6k and 4E-BP1 was found in dystrophic muscles treated with ADMSC. These results suggest that ADMSC transplantation modulates inflammation and improves muscle tissue regeneration, ameliorating the dystrophic phenotype in dystrophin-deficient mice. |
| Databáze: | OpenAIRE |
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