Multiplex recording of cellular events over time on CRISPR biological tape

Autor:	Felix L. Wu, Sung Sun Yim, Ravi U. Sheth, Harris H. Wang
Rok vydání:	2017
Předmět:	0301 basic medicine Multiple days business.product_category Cells Population Information Storage and Retrieval Nanotechnology Computational biology Biology Multiplexing Genome Article 03 medical and health sciences Tape recorder 0302 clinical medicine CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Multiplex education Fucose Oligonucleotide Array Sequence Analysis education.field_of_study Multidisciplinary Trehalose DNA CRISPR Arrays 030104 developmental biology CRISPR-Cas Systems business Copper 030217 neurology & neurosurgery
Zdroj:	Science
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.aao0958
Popis:	A CRISPR device to record time The CRISPR adaptation system has been used to record the sequence and ordering of exogenous oligonucleotides that are electroporated into cell populations. Sheth et al. engineered a system bypassing the use of exogenous DNA to directly record temporal signals. An input biological signal is transformed into the ratio of the frequency of incorporating trigger DNA to that of incorporating reference DNA into the genomes of a bacterial population. A multiplexing strategy enables simultaneous recording of three environmental signals with high temporal resolution. Science , this issue p. 1457
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad049d02eed16ce797865330c7fd1813 https://doi.org/10.1126/science.aao0958 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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