Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk
| Autor: | Cunyi Zhao, Yang Si, Gang Sun, Thierry Noguer, Ahmed Y. El-Moghazy, Noha Amaly, Georges Istamboulie |
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| Rok vydání: | 2018 |
| Předmět: |
Surface Properties
Nanofibers Biomedical Engineering Biophysics Biosensing Techniques 02 engineering and technology Electrochemistry 01 natural sciences Electron Transport Limit of Detection Animals Immunoassay Detection limit Chromatography integumentary system Chemistry 010401 analytical chemistry Membranes Artificial Electrochemical Techniques General Medicine 021001 nanoscience & nanotechnology Drug Residues Amperometry Anti-Bacterial Agents 0104 chemical sciences Dielectric spectroscopy Chloramphenicol Milk Membrane Polyethylene Dielectric Spectroscopy Polyvinyl Alcohol Nanofiber Electrode 0210 nano-technology Selectivity Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 117:838-844 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.07.025 |
| Popis: | An ultrasensitive label-free amperometric immunosensor for the detection of chloramphenicol (CAP) residues in milk has been developed by using a screen-printed carbon electrode laminated with a layer of poly (vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane that is covalently immobilized with a CAP antibody (anti-CAP). The performance of the PVA-co-PE nanofiber membrane (PVA-co-PE NFM) on the electrode was compared with a PVA-co-PE casted membrane (PVA-co-PE CM), necessary fabrication steps and performance of the sensors were investigated by electrochemical impedance spectroscopy (EIS). The application of the PVA-co-PE NFM decreased the electron-transfer-resistance by about 4 times compared with a conventional PVA-co-PE casted membrane. Under the optimal conditions, the established immunosensor exhibited high sensitivity for determination of CAP in a range 0.01–10 ng mL−1, with a limit of detection of 0.0047 ng mL−1. In addition to the good selectivity, reusability and stability over time, the prepared immunosensor was successfully used in the detection of CAP in milk samples without any pretreatment. |
| Databáze: | OpenAIRE |
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