Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression

Autor: Raquel F. Carvalho, Paula Duque, Dale N. Richardson, Tiago M. D. Cruz
Jazyk: angličtina
Rok vydání: 2014
Předmět:
0106 biological sciences
Arabidopsis thaliana
alternative splicing (AS)
Arabidopsis
ABA-responsive cis elements
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Gene Expression Regulation
Plant
Gene expression
Phosphoprotein Phosphatases
Regulatory Elements
Transcriptional
lcsh:QH301-705.5
Abscisic acid
Spectroscopy
Genetics
0303 health sciences
biology
food and beverages
General Medicine
ABI1
Computer Science Applications
abscisic acid (ABA)
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
SR protein
RNA
Messenger
Physical and Theoretical Chemistry
Molecular Biology
Gene
030304 developmental biology
Arabidopsis Proteins
Organic Chemistry
Alternative splicing
fungi
biology.organism_classification
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Seedlings
gene expression
SR proteins
010606 plant biology & botany
Abscisic Acid
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
International Journal of Molecular Sciences
Volume 15
Issue 10
Pages 17541-17564
International Journal of Molecular Sciences, Vol 15, Iss 10, Pp 17541-17564 (2014)
Popis: Serine/arginine-rich (SR) proteins are major modulators of alternative splicing, a key generator of proteomic diversity and flexible means of regulating gene expression likely to be crucial in plant environmental responses. Indeed, mounting evidence implicates splicing factors in signal transduction of the abscisic acid (ABA) phytohormone, which plays pivotal roles in the response to various abiotic stresses. Using real-time RT-qPCR, we analyzed total steady-state transcript levels of the 18 SR and two SR-like genes from Arabidopsis thaliana in seedlings treated with ABA and in genetic backgrounds with altered expression of the ABA-biosynthesis ABA2 and the ABA-signaling ABI1 and ABI4 genes. We also searched for ABA-responsive cis elements in the upstream regions of the 20 genes. We found that members of the plant-specific SC35-Like (SCL) Arabidopsis SR protein subfamily are distinctively responsive to exogenous ABA, while the expression of seven SR and SR-related genes is affected by alterations in key components of the ABA pathway. Finally, despite pervasiveness of established ABA-responsive promoter elements in Arabidopsis SR and SR-like genes, their expression is likely governed by additional, yet unidentified cis-acting elements. Overall, this study pinpoints SR34, SR34b, SCL30a, SCL28, SCL33, RS40, SR45 and SR45a as promising candidates for involvement in ABA-mediated stress responses. FCT PostDoctoral Fellowships: SFRH/BPD/80073/2011, SFRH/BPD/81830/2011.
Databáze: OpenAIRE
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