The effect of biodegradable silk fibroin-based scaffolds containing glial cell line-derived neurotrophic factor (GDNF) on the corneal regeneration process
| Autor: | S.A. Borzenok, O. I. Agapova, A.V. Revishchin, M. M. Bobrova, L. A. Safonova, M.B. Agammedov, A. E. Efimov, D.S. Ostrovkiy, G.V. Pavlova, N.A. Gavrilova, O.E. Tishchenko, I. I. Agapov |
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| Rok vydání: | 2021 |
| Předmět: |
Keratinocytes
Male Stromal cell Primary Cell Culture Fibroin 02 engineering and technology Biochemistry Mice 03 medical and health sciences Structural Biology Cornea medicine Glial cell line-derived neurotrophic factor Animals Humans Glial Cell Line-Derived Neurotrophic Factor Gap-43 protein Molecular Biology Cell Proliferation 030304 developmental biology 0303 health sciences Tissue Scaffolds biology Chemistry Regeneration (biology) fungi Nerve plexus General Medicine 021001 nanoscience & nanotechnology Cell biology medicine.anatomical_structure biology.protein Immunohistochemistry Fibroins 0210 nano-technology Biomarkers Corneal Injuries |
| Zdroj: | International Journal of Biological Macromolecules. 185:264-276 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.06.040 |
| Popis: | Corneal injury due to ocular trauma or infection is one of the most challenging vision impairing pathologies. The aim of the work was to study the effect of biodegradable silk fibroin-based scaffolds containing GDNF on the corneal regeneration process. During cultivate the highest keratocytes proliferative activity was registered with scaffolds containing 250 ng/ml and 500 ng/ml GDNF. In mice with an experimental model of epithelial-stromal damage to the cornea, silk fibroin-based scaffolds containing GDNF in various concentrations were used (in groups 1, 2 and 3 silk fibroin-based scaffolds containing GDNF in a concentration of 50 ng/ml, 250 ng/ml and 500 ng/ml, respectively; in group 4 - silk fibroin-based scaffolds without GDNF; in group 5 - a solution of GDNF with concentration of 500 ng/ml; group 6- control). The area of the corneal epithelial defect in groups 2, 3, and 5 was less than in the other groups. The most pronounced positive immunohistochemical reaction with antibodies to Bcl2, Bax, phosphoERK1/2 and phospho-JNK1/2, Ki67, Gap43 was observed in groups 2 and 3. Thus, silk fibroin-based scaffolds with GDNF stimulate the epithelialization process, proliferative activity of epithelial cells and keratocytes, accelerate the formation of the stromal nerve plexus and exhibit anti-apoptotic activity. |
| Databáze: | OpenAIRE |
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