Low-level laser therapy influences mouse odontoblast-like cell response in vitro
| Autor: | Luciano Bachmann, Daniela Thomazatti Chimello, Adalberto Luiz Rosa, Maidy Rehder Wimmers Ferreira, Karina Fittipaldi Bombonato-Prado, Luciana Batista Pereira |
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| Rok vydání: | 2012 |
| Předmět: |
Vascular Endothelial Growth Factor A
Pathology medicine.medical_specialty Time Factors Cell Survival medicine.medical_treatment Biomedical Engineering Enzyme-Linked Immunosorbent Assay Biology In Vitro Techniques Radiation Dosage Real-Time Polymerase Chain Reaction chemistry.chemical_compound Mice Random Allocation stomatognathic system Reference Values ODONTOBLASTO (CRESCIMENTO E DESENVOLVIMENTO) medicine Animals Radiology Nuclear Medicine and imaging Viability assay Low-Level Light Therapy Low level laser therapy Cells Cultured Cell Proliferation Odontoblasts Molecular biology In vitro DMP1 Vascular endothelial growth factor Odontoblast chemistry Pulp (tooth) Alkaline phosphatase |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1557-8550 |
| Popis: | The purpose of this study was to analyze the influence of two different irradiation times with 85 mW/cm(2) 830 nm laser on the behavior of mouse odontoblast-like cells.The use of low-level laser therapy (LLLT) to stimulate pulp tissue is a reality, but few reports relate odontoblastic responses to irradiation in in vitro models.Odontoblast-like cells (MDPC-23) were cultivated and divided into three groups: control/nonirradiated (group 1); or irradiated with 85 mW/cm(2), 830 nm laser for 10 sec (0.8 J/cm(2)) (group 2); or for 50 sec (4.2 J/cm(2)) (group 3) with a wavelength of 830 nm. After 3, 7, and 10 days, it was analyzed: growth curve and cell viability, total protein content, alkaline phosphatase (ALP) activity, calcified nodules detection and quantification, collagen immunolocalization, vascular endothelial growth factor (VEGF) expression, and real-time polymerase chain reaction (PCR) for DMP1 gene. Data were analyzed by Kruskall-Wallis test (α=0.05).Cell growth was smaller in group 2 (p0.01), whereas viability was similar in all groups and at all periods. Total protein content and ALP activity increased on the 10th day with 0.8 J/cm(2) (p0.01), as well as the detection and quantification of mineralization nodules (p0.05), collagen, and VEGF expression (p0.01). The expression of DMP1 increased in all groups (p0.05) compared with control at 3 days, except for 0.8 J/cm(2) at 3 days and control at 10 days.LLLT influenced the behavior of odontoblast-like cells; the shorter time/smallest energy density promoted the expression of odontoblastic phenotype in a more significant way. |
| Databáze: | OpenAIRE |
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