Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers
| Autor: | Suzana Erico Tanni, Simone Alves do Vale, Aparecida Y.O. Angeleli, Liana Sousa Coelho, Irma Godoy, Camila Renata Corrêa |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Allergy Clinical Biochemistry Oxidative phosphorylation medicine.disease_cause Systemic inflammation chemistry.chemical_compound Internal medicine medicine Hydrogen peroxide business.industry Research lcsh:RM1-950 Smoking Cell Biology medicine.disease Peripheral blood Intensity (physics) Cultured cells lcsh:Therapeutics. Pharmacology Endocrinology chemistry Oxidative stress Tobacco exposure Immunology medicine.symptom business |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Inflammation (London, England) Journal of Inflammation, Vol 9, Iss 1, p 45 (2012) |
| ISSN: | 0003-1259 |
| Popis: | Made available in DSpace on 2013-08-28T14:07:39Z (GMT). No. of bitstreams: 1 WOS000312597300001.pdf: 243329 bytes, checksum: a878d23efe1c0603a1ac0d918268fbc0 (MD5) Made available in DSpace on 2013-09-30T18:22:56Z (GMT). No. of bitstreams: 2 WOS000312597300001.pdf: 243329 bytes, checksum: a878d23efe1c0603a1ac0d918268fbc0 (MD5) WOS000312597300001.pdf.txt: 29136 bytes, checksum: 835b2fc3c1ce0cf53ee478a2292b1bde (MD5) Previous issue date: 2012-11-21 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:33:13Z No. of bitstreams: 2 WOS000312597300001.pdf: 243329 bytes, checksum: a878d23efe1c0603a1ac0d918268fbc0 (MD5) WOS000312597300001.pdf.txt: 29136 bytes, checksum: 835b2fc3c1ce0cf53ee478a2292b1bde (MD5) Made available in DSpace on 2014-05-20T13:33:13Z (GMT). No. of bitstreams: 2 WOS000312597300001.pdf: 243329 bytes, checksum: a878d23efe1c0603a1ac0d918268fbc0 (MD5) WOS000312597300001.pdf.txt: 29136 bytes, checksum: 835b2fc3c1ce0cf53ee478a2292b1bde (MD5) Previous issue date: 2012-11-21 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers.Methods: A total of 25 smokers (50.3 +/- 8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-alpha were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1 +/- 8.2 years, 42% male).Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|