Mandibular Appliance Modulates Condylar Growth through Integrins
| Autor: | Denise Hajjar, M. Fagundes Santos, M. Rubia Marques, K. Gomes Franchini, A. Sigari Moriscot |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Integrins medicine.medical_specialty Integrin Integrin alpha5 Mandible Mechanotransduction Cellular Chondrocytes stomatognathic system Insulin-Like Growth Factor II Internal medicine Gene expression medicine Animals Amino Acid Sequence Insulin-Like Growth Factor I Rats Wistar Receptors Immunologic General Dentistry Cells Cultured Cell Proliferation Messenger RNA biology Chemistry Cell growth Cartilage Integrin alphaV Immunohistochemistry Biomechanical Phenomena Fibronectins Rats Fibronectin medicine.anatomical_structure Endocrinology biology.protein Orthodontic Appliances Functional Stress Mechanical Oligopeptides |
| Zdroj: | Journal of Dental Research. 87:153-158 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/154405910808700210 |
| Popis: | Functional orthopedic therapy corrects growth discrepancies between the maxilla and mandible, possibly through postural changes in the musculature and modulation of the mandibular condylar cartilage growth. Using Wistar rats, we tested the hypothesis that chondrocytes respond to forces generated by a mandibular propulsor appliance by changes in gene expression, and that integrins are important mediators in this response. Immunohistochemical analyses demonstrated that the use of the appliance for different periods of time modulated the expression of fibronectin, α5 and αv integrin subunits, as well as cell proliferation in the cartilage. In vitro, cyclic distension of condylar cartilage-derived cells increased fibronectin mRNA, as well as Insulin-like Growth Factor-I and II mRNA and cell proliferation. A peptide containing the Arginine-Glycine-Asparagine sequence (RGD), the main cell-binding sequence in fibronectin, blocked almost all these effects, confirming that force itself modulates the growth of the rat condylar cartilage, and that RGD-binding integrins participate in mechanotransduction. |
| Databáze: | OpenAIRE |
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