Determination of Ochratoxin A in Green Coffee by Immunoaffinity Column Cleanup and Liquid Chomatography: Collaborative Study
| Autor: | Mallmann Ca, Taniwaki Mh, dos Santos Ea, G. A. Lombaert, Steiner W, Patel S, Neil Rj, Wee Sm, Susan MacDonald, Eugenia Azevedo Vargas, Diaz Gj, Petracco M, A Pittet, J. Stroka, Koch P, Meier P, Gorni R, Corrêa Tb, Myrna Sabino, Prado G, da Rocha Ap, Nakajima M |
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| Rok vydání: | 2005 |
| Předmět: |
Ochratoxin A
Time Factors Relative standard deviation Food Contamination Buffers Sodium Chloride Coffee Chemistry Techniques Analytical Chromatography Affinity Phosphates Analytical Chemistry chemistry.chemical_compound Environmental Chemistry media_common.cataloged_instance European union Green coffee media_common Pharmacology Reproducibility Chromatography Chemistry Elution Methanol Reproducibility of Results Repeatability Contamination Ochratoxins Sodium Bicarbonate Spectrometry Fluorescence Calibration Agronomy and Crop Science Food Analysis Chromatography Liquid Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 88:773-779 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/88.3.773 |
| Popis: | A collaborative study was conducted to evaluate a method using immunoaffinity column cleanup with liquid chromatography (LC) for the determination of ochratoxin A (OTA) in green coffee at levels that could be included in possible future regulations of the European Union. The test portion was extracted with methanol–3% aqueous sodium hydrogen carbonate solution (50 + 50, v/v). The extract was filtered, and the filtrate was diluted with phosphate-buffered saline and applied to an immunoaffinity column containing antibodies specific for OTA. After washing, the toxin was eluted from the column with methanol and quantified by LC with fluorescence detection. Pairs of 4 homogeneous noncontaminated and naturally contaminated materials (mean levels of |
| Databáze: | OpenAIRE |
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