Two active forms of RD-114 virus DNA polymerase in infected cells
| Autor: | Paul T. Peebles, Susan G. Smith, Brenda I. Gerwin |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Poly T
DNA polymerase viruses DNA polymerase II General Biochemistry Genetics and Molecular Biology Virus Cell Line biology.animal Magnesium Mink Polymerase chemistry.chemical_classification Chromatography Manganese DNA clamp biology Cell-Free System RNA-Directed DNA Polymerase Templates Genetic Virology Molecular biology Isoenzymes Molecular Weight Enzyme Retroviridae chemistry Cell culture biology.protein Poly A |
| Zdroj: | Cell. 6(1) |
| ISSN: | 0092-8674 |
| Popis: | Two forms of DNA polymerase are present in RD-114-infected human, dog, and mink cells, but are not detectable in uninfected cells. The two enzymes are indistinguishable catalytically and immunologically, but differ with respect to molecular weight and elution position from (dT)12-18-cellulose and phosphocellulose. The large enzyme (equivalent 95,000 daltons) is found in the infected cells, but not the virions produced by these cells. The virions contain only the smaller enzyme (equivalent 70,000 daltons). The larger form may represent a mammalian viral equivalent to the beta subunit of avian RNA tumor virus DNA polymerase. |
| Databáze: | OpenAIRE |
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